Katanaev Vladimir L, Tomlinson Andrew
Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, Suite 1120, New York, NY 10032, USA.
Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6524-9. doi: 10.1073/pnas.0601853103. Epub 2006 Apr 14.
During asymmetric division, a cell polarizes and differentially distributes components to its opposite ends. The subsequent division differentially segregates the two component pools to the daughters, which thereby inherit different developmental directives. In Drosophila sensory organ precursor cells, the localization of Numb protein to the cell's anterior cortex is a key patterning event and is achieved by the combined action of many proteins, including Pins, which itself is localized anteriorly. Here, a role is described for the trimeric G protein Go in the anterior localization of Numb and daughter cell fate specification. Go is shown to interact with Pins. In addition to a role in recruiting Numb to an asymmetric location in the cell's cortex, Go transduces a signal from the Frizzled receptor that directs the position in which the complex forms. Thus, Go likely integrates the signaling that directs the formation of the complex with the signaling that directs where the complex forms.
在不对称分裂过程中，细胞发生极化并将成分差异地分布到其相对的两端。随后的分裂将两个成分库差异地分离到子细胞中，从而使子细胞继承不同的发育指令。在果蝇感觉器官前体细胞中，Numb蛋白定位于细胞前皮质是一个关键的模式形成事件，这是由许多蛋白质的共同作用实现的，包括本身定位于前部的Pins。本文描述了三聚体G蛋白Go在Numb的前定位和子细胞命运决定中的作用。研究表明Go与Pins相互作用。除了在将Numb招募到细胞皮质的不对称位置中发挥作用外，Go还转导来自卷曲受体的信号，该信号指导复合物形成的位置。因此，Go可能将指导复合物形成的信号与指导复合物形成位置的信号整合在一起。
