Expression of in the Olfactory System of Embryonic Zebrafish ().

The Reprimo () family is composed of highly conserved single-exon genes. The expression pattern of this gene family has been recently described during zebrafish () embryogenesis, and primarily locates in the nervous system. Its most characterized member, , which duplicated to give rise and in the fish lineage, is known to act as a tumor-suppressor gene in mammalian models. Here, we describe in detail the spatiotemporal expression of three genes (, and l) within distinct anatomical structures in the developing peripheral and central nervous system. In the zebrafish, mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. Most importantly, the expression of has been conserved between teleosts and mammals. Thus, we provide a reference dataset describing the expression patterns of gene products during zebrafish and mouse development as a first step to approach the physiological role of the gene family.