Wang Xinyu, Xing Defeng, Mei Xiaoxue, Liu Bingfeng, Ren Nanqi
State Key Laboratory of Urban Water Resources and Environment, School of Environment, Harbin Institute of Technology, Harbin, China.
Front Microbiol. 2018 Mar 27;9:580. doi: 10.3389/fmicb.2018.00580. eCollection 2018.
-Nitrophenol (PNP) is common in the wastewater from many chemical industries. In this study, we investigated the effect of initial concentrations of PNP and glucose and applied voltage on PNP reduction in biocathode BESs and open-circuit biocathode BESs (OC-BES). The PNP degradation efficiency of a biocathode BES with 0.5 V (Bioc-0.5) reached 99.5 ± 0.8%, which was higher than the degradation efficiency of the BES with 0 V (Bioc-0) (62.4 ± 4.5%) and the OC-BES (59.2 ± 12.5%). The PNP degradation rate constant () of Bioc-0.5 was 0.13 ± 0.01 h, which was higher than the of Bioc-0 (0.024 ± 0.002 h) and OC-BES (0.013 ± 0.0005 h). PNP degradation depended on the initial concentrations of glucose and PNP. A glucose concentration of 0.5 g L was best for PNP degradation. The initial PNP increased from 50 to 130 mg L and the decreased from 0.093 ± 0.008 to 0.027 ± 0.001 h. High-throughput sequencing of 16S rRNA gene amplicons indicated differences in microbial community structure between BESs with different voltages and the OC-BES. The predominant populations were affiliated with (42.7%) and (54.1%) in biocathode biofilms of BESs, and were the predominant microorganisms in biocathode biofilms of OC-BESs. The predominant populations were different among the cathode biofilms and the suspensions. These results demonstrated that applied voltage and biocathode biofilms play important roles in PNP degradation.
对硝基苯酚（PNP）在许多化工行业的废水中很常见。在本研究中，我们研究了PNP和葡萄糖的初始浓度以及施加电压对生物阴极微生物电解池（BESs）和开路生物阴极微生物电解池（OC - BES）中PNP还原的影响。施加0.5 V电压的生物阴极BES（Bioc - 0.5）对PNP的降解效率达到99.5±0.8%，高于施加0 V电压的BES（Bioc - 0）（62.4±4.5%）和OC - BES（59.2±12.5%）的降解效率。Bioc - 0.5的PNP降解速率常数（）为0.13±0.01 h，高于Bioc - 0（0.024±0.002 h）和OC - BES（0.013±0.0005 h）。PNP降解取决于葡萄糖和PNP的初始浓度。葡萄糖浓度为0.5 g/L时最有利于PNP降解。初始PNP浓度从50 mg/L增加到130 mg/L时，降解速率常数从0.093±0.008 h降至0.027±0.001 h。16S rRNA基因扩增子的高通量测序表明，不同电压的BESs与OC - BES之间微生物群落结构存在差异。在BESs的生物阴极生物膜中，优势菌群隶属于（42.7%）和（54.1%），而在OC - BESs的生物阴极生物膜中，是优势微生物。阴极生物膜和悬浮液中的优势菌群不同。这些结果表明，施加电压和生物阴极生物膜在PNP降解中起重要作用。
