Faria-Blanc Nuno, Mortimer Jenny C, Dupree Paul
Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
Front Plant Sci. 2018 Mar 27;9:384. doi: 10.3389/fpls.2018.00384. eCollection 2018.
Yeast have long been known to possess a cell wall integrity (CWI) system, and recently an analogous system has been described for the primary walls of plants (PCWI) that leads to changes in plant growth and cell wall composition. A similar system has been proposed to exist for secondary cell walls (SCWI). However, there is little data to support this. Here, we analyzed the stem transcriptome of a set of cell wall biosynthetic mutants in order to investigate whether cell wall damage, in this case caused by aberrant xylan synthesis, activates a signaling cascade or changes in cell wall synthesis gene expression. Our data revealed remarkably few changes to the transcriptome. We hypothesize that this is because cells undergoing secondary cell wall thickening have entered a committed programme leading to cell death, and therefore a SCWI system would have limited impact. The absence of transcriptomic responses to secondary cell wall alterations may facilitate engineering of the secondary cell wall of plants.
长期以来，人们都知道酵母拥有细胞壁完整性（CWI）系统，最近还描述了一种类似的系统，即植物初生壁（PCWI）系统，该系统会导致植物生长和细胞壁组成发生变化。有人提出次生细胞壁（SCWI）也存在类似系统。然而，几乎没有数据支持这一点。在这里，我们分析了一组细胞壁生物合成突变体的茎转录组，以研究在这种情况下由异常木聚糖合成引起的细胞壁损伤是否会激活信号级联反应或改变细胞壁合成基因的表达。我们的数据显示转录组的变化非常少。我们推测这是因为经历次生细胞壁加厚的细胞已经进入了导致细胞死亡的既定程序，因此SCWI系统的影响有限。对次生细胞壁改变缺乏转录组反应可能有助于对植物次生细胞壁进行工程改造。
