Greimers R, Defresne M P, Rongy A M, Humblet C, Boniver J
Laboratoire d'Anatomie Pathologique, C. H. U. B23, Université de Liège au Sart Tilman, Liège.
C R Seances Soc Biol Fil. 1987;181(4):420-5.
The ploidy of the thymus was studied in C57B1/Ka mice irradiated with 4 weekly X-Ray doses of 1.75 Gy. The determination of nuclear DNA content was performed by flow cytometry of intact thymocytes labeled with propidium iodide in presence of a mixture of chicken and rainbow trout red blood cells as internal reference standards. The method has been tested by detecting the sex difference in DNA content of G0/G1 of normal thymic mouse cells. The mean value was 2.9% higher in female mice. The thymus of almost 60% of irradiated male mice present a slight hypoploidy of 2.6% one month after the last irradiation.
对接受每周 4 次、每次 1.75 Gy X 射线照射的 C57B1/Ka 小鼠的胸腺倍性进行了研究。通过流式细胞术测定完整胸腺细胞的核 DNA 含量，这些胸腺细胞用碘化丙啶标记，并以鸡和虹鳟红细胞的混合物作为内参标准。该方法已通过检测正常胸腺小鼠细胞 G0/G1期 DNA 含量的性别差异进行了测试。雌性小鼠的平均值高 2.9%。在最后一次照射后一个月，近 60% 接受照射的雄性小鼠的胸腺出现了 2.6% 的轻微亚倍体现象。
