Biophotonic Department, Universidade Nove de Julho, São Paulo, SP, Brazil.
Cardiology Division, Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Oxid Med Cell Longev. 2018 Feb 13;2018:5763256. doi: 10.1155/2018/5763256. eCollection 2018.
The aim of this study was to determine whether oxidative stress markers are influenced by low-intensity laser therapy (LLLT) in rats subjected to a high-intensity resistive exercise session (RE). Female Wistar rats divided into three experimental groups (Ctr: control, 4J: LLLT, and RE) and subdivided based on the sampling times (instantly or 24 h postexercise) underwent irradiation with LLLT using three-point transcutaneous method on the hind legs, which was applied to the gastrocnemius muscle at the distal, medial, and proximal points. Laser (4J) or placebo (device off) were carried out 60 sec prior to RE that consisted of four climbs bearing the maximum load with a 2 min time interval between each climb. Lipoperoxidation levels and antioxidant capacity were obtained in muscle. Lipoperoxidation levels were increased (4-HNE and CL markers) instantly post-RE. LLLT prior to RE avoided the increase of the lipid peroxidation levels. Similar results were also notified for oxidation protein assays. The GPx and FRAP activities did not reduce instantly or 24 h after RE. SOD increased 24 h after RE, while CAT activity did not change with RE or LLLT. In conclusion, LLLT prior to RE reduced the oxidative stress markers, as well as, avoided reduction, and still increased the antioxidant capacity.
本研究旨在确定低强度激光疗法（LLLT）是否会影响高强度抗阻运动（RE）后大鼠的氧化应激标志物。雌性 Wistar 大鼠分为三组（Ctr：对照组、4J：LLLT 组和 RE 组），并根据采样时间（运动即刻或运动后 24 小时）进一步分为亚组，采用三点经皮法对后腿进行 LLLT 照射，将激光（4J）或安慰剂（设备关闭）照射到腓肠肌的远端、内侧和近端三个点。RE 前 60 秒进行 LLLT，RE 包括四次最大负荷攀爬，每次攀爬之间间隔 2 分钟。在肌肉中获得脂质过氧化水平和抗氧化能力。RE 后即刻，脂类过氧化水平（4-HNE 和 CL 标志物）升高。RE 前进行 LLLT 可避免脂质过氧化水平升高。氧化蛋白检测也得到了类似的结果。RE 后即刻或 24 小时，GPx 和 FRAP 活性没有降低。SOD 在 RE 后 24 小时增加，而 CAT 活性不受 RE 或 LLLT 影响。总之，RE 前进行 LLLT 可降低氧化应激标志物，并避免减少，同时还能增加抗氧化能力。
