National Key Laboratory of Photoelectric Technology, Functional Materials (Culture Base) in Shaanxi Province, National Photoelectric Technology, Functional Materials & Application of Science and Technology International Cooperation Base, Institute of Photonics & Photon-Technology , Northwest University , Xi'an 710069 , China.
Department of Pharmaceutical Analysis, School of Pharmacy, and the State Key Laboratory of Cancer Biology (CBSKL) , The Fourth Military Medical University , 169th Changle West Road , Xi'an , Shaanxi 710032 , China.
ACS Appl Mater Interfaces. 2018 May 2;10(17):14570-14576. doi: 10.1021/acsami.8b03239. Epub 2018 Apr 19.
A difunctional nano-photothermal therapy (PTT) platform with near-infrared excitation to near-infrared emission (NIR-to-NIR) was constructed through core-shell structures YO:Nd/Yb@SiO@CuS (YRSC), in which the core YO:Nd/Yb and shell CuS play the role of bioimaging and photothermal conversion function, respectively. The structure and composition of the present PTT agents (PTAs) were characterized by powder X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, and X-ray photoelectron spectra. The NIR emissions of samples in the biological window area were measured by photoluminescence spectra under the excitation of 808 nm laser; further, the penetration depth of NIR emission at different wavelengths in biological tissue was also demonstrated by comparing with visible (vis) emission from YO:Yb/Er@SiO@CuS and NIR emission from YRSC through different injection depths in pork muscle tissues. The photo-thermal conversion effects were achieved through the outer ultrasmall CuS nanoparticles simultaneously absorb NIR light emission from the core YO:NdYb and the 808 nm excitation source to generate heat. Further, the heating effect of YRSC nanoparticles was confirmed by thermal imaging and ablation of YRSC to Escherichia coli and human hepatoma (HepG-2) cells. Results indicate that the YRSC has potential applications in PTT and NIR imaging in biological tissue.
构建了一种具有近红外激发至近红外发射（NIR-to-NIR）功能的双功能纳米光热治疗（PTT）平台，该平台采用核壳结构 YO:Nd/Yb@SiO@CuS（YRSC），其中核 YO:Nd/Yb 和壳 CuS 分别发挥生物成像和光热转换功能。通过粉末 X 射线衍射、场发射扫描电子显微镜、透射电子显微镜和 X 射线光电子能谱对本 PTT 试剂（PTAs）的结构和组成进行了表征。在 808nm 激光激发下，通过光致发光光谱测量了样品在生物窗口区域的近红外发射；此外，还通过比较猪肉组织中不同注入深度的 YO:Yb/Er@SiO@CuS 的可见（vis）发射和 YRSC 的近红外发射，证明了不同波长近红外发射在生物组织中的穿透深度。通过外壳的超小 CuS 纳米粒子同时吸收来自核 YO:NdYb 的近红外发光和 808nm 激发源来产生热量，从而实现光热转换效果。进一步通过热成像和 YRSC 对大肠杆菌和人肝癌（HepG-2）细胞的消融来证实了 YRSC 纳米粒子的加热效果。结果表明，YRSC 在生物组织中的 PTT 和近红外成像中有潜在的应用。
