Tati Ramesh, Nordin Sara, Abdillahi Suado M, Mörgelin Matthias
Postdoctoral Researcher, Department of Clinical Sciences, Division of Infection Medicine, Lund University, SE-221 84 Lund, Sweden.
Department of Clinical Sciences, Division of Infection Medicine, Lund University, SE-221 84 Lund, Sweden, Colzyx ltd, Medicon Village, Scheelevägen 2, SE-223 81 Lund, Sweden.
J Wound Care. 2018 Apr 2;27(4):199-209. doi: 10.12968/jowc.2018.27.4.199.
When the delicate balance between catabolic and anabolic processes is disturbed for any reason, the healing process can stall, resulting in chronic wounds. In chronic wound pathophysiology, proteolytic imbalance is implicated due to elevated protease levels mediating tissue damage. Hence, it is important to design appropriate wound treatments able to control and modulate protease activity directly at the host/biomaterial interface. Here, we investigate collagen-based wound dressings with the focus on their potential to adsorb and inactivate tissue proteases.
We examined the effect of six collagen-based dressings on their ability to adsorb and inactivate different granulocyte proteases, plasmin, human neutrophil elastase (HLE), and matrix metalloproteases (MMP)-1, -2, -8, and -9, by an integrated approach including immunoelectron microscopy.
We observed a reduction of the proteolytic activities of plasmin, HLE, and MMP-1, -2, -8, and -9, both on the biomaterial surface and in human chronic wound fluid. The most pronounced effect was observed in collagen-based dressings, with the highest content of native collagen networks resembling dermis structures.
Our data suggest that this treatment strategy might be beneficial for the chronic wound environment, with the potential to promote improved wound healing.
当分解代谢和合成代谢过程之间的微妙平衡因任何原因受到干扰时，愈合过程可能会停滞，导致慢性伤口。在慢性伤口病理生理学中，由于介导组织损伤的蛋白酶水平升高，蛋白水解失衡被认为与之相关。因此，设计能够在宿主/生物材料界面直接控制和调节蛋白酶活性的合适伤口治疗方法非常重要。在此，我们研究基于胶原蛋白的伤口敷料，重点关注其吸附和灭活组织蛋白酶的潜力。
我们通过包括免疫电子显微镜在内的综合方法，研究了六种基于胶原蛋白的敷料对其吸附和灭活不同粒细胞蛋白酶、纤溶酶、人中性粒细胞弹性蛋白酶（HLE）以及基质金属蛋白酶（MMP）-1、-2、-8和-9的能力的影响。
我们观察到，在生物材料表面和人类慢性伤口液中，纤溶酶、HLE以及MMP-1、-2、-8和-9的蛋白水解活性均有所降低。在基于胶原蛋白的敷料中观察到最显著的效果，这些敷料中天然胶原网络的含量最高，类似于真皮结构。
我们的数据表明，这种治疗策略可能对慢性伤口环境有益，具有促进伤口愈合改善的潜力。
