Snell J J, Brown D F
Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, Colindale, London.
J Clin Pathol. 1988 Jan;41(1):97-102. doi: 10.1136/jcp.41.1.97.
Six strains of Neisseria gonorrhoeae were distributed to 411 United Kingdom laboratories who were asked to test the susceptibility of the strains to penicillin, cefuroxime, tetracycline and spectinomycin and to test for production of beta-lactamase. Details of methods used were requested by means of a questionnaire. The number of reports recording sensitive strains as resistant was 5% for penicillin, 0.7% for cefuroxime, 3% for tetracycline and 4% for spectinomycin. The number of reports recording resistant strains as sensitive was 7% for penicillin (0.2% with beta-lactamase producing strains, 20% with non-beta-lactamase producing strains), 96% for cefuroxime, 76% for tetracycline and 8% for spectinomycin. There was an association between greater error rates and the use of high content discs for testing tetracycline, the use of low content discs for testing spectinomycin, failure to dilute the inoculum, and use of acidimetric methods rather than methods that use a chromogenic cephalosporin for detecting beta-lactamase.
六株淋病奈瑟菌被分发给411家英国实验室，要求这些实验室检测这些菌株对青霉素、头孢呋辛、四环素和壮观霉素的敏感性，并检测β-内酰胺酶的产生情况。通过问卷调查的方式获取所使用方法的详细信息。将敏感菌株记录为耐药菌株的报告比例，青霉素为5%，头孢呋辛为0.7%，四环素为3%，壮观霉素为4%。将耐药菌株记录为敏感菌株的报告比例，青霉素为7%（产β-内酰胺酶菌株为0.2%，非产β-内酰胺酶菌株为20%），头孢呋辛为96%，四环素为76%，壮观霉素为8%。错误率较高与使用高含量四环素检测盘、使用低含量壮观霉素检测盘、未稀释接种物以及使用酸碱滴定法而非使用显色头孢菌素检测β-内酰胺酶的方法之间存在关联。
