NIBIO, Norwegian Institute of Bioeconomy Research, Hoghskoleveien 7, N-1431 Aas, Norway.
NIBIO, Norwegian Institute of Bioeconomy Research, Svanhovd, N-9925 Svanvik, Norway.
Molecules. 2018 Apr 8;23(4):850. doi: 10.3390/molecules23040850.
The apple fruit moth (Lepidoptera, Yponomeutidae) is a seed predator of rowan () and is distributed in Europe and Asia. In Fennoscandia (Finland, Norway and Sweden), rowan fruit production is low every 2-4 years, and apple () functions as an alternative host, resulting in economic loss in apple crops in inter-mast years. We have used Illumina MiSeq sequencing to identify a set of 19 novel tetra-nucleotide short tandem repeats (STRs) in . Such motifs are recommended for genetic monitoring, which may help to determine the eco-evolutionary processes acting on this pest insect. The 19 STRs were optimized and amplified into five multiplex PCR reactions. We tested individuals collected from Norway and Sweden ( = 64), and detected very high genetic variation (average 13.6 alleles, He = 0.75) compared to most other Lepidoptera species studied so far. Spatial genetic differentiation was low and gene flow was high in the test populations, although two non-spatial clusters could be detected. We conclude that this set of genetic markers may be a useful resource for population genetic monitoring of this economical important insect species.
苹果实蛾（鳞翅目，果蛾科）是一种危害花楸果实的幼虫，分布于欧洲和亚洲。在芬诺斯堪的亚（芬兰、挪威和瑞典），花楸果实的产量每 2-4 年就会出现一次低谷，而苹果是其替代宿主，这导致了苹果作物在结实间隔年的经济损失。我们利用 Illumina MiSeq 测序技术在苹果实蛾中鉴定出了一组 19 个新的四核苷酸短串联重复（STR）。这些基序被推荐用于遗传监测，这可能有助于确定作用于这种害虫的生态进化过程。我们对从挪威和瑞典采集的个体（n = 64）进行了优化和五重 PCR 扩增，与迄今为止研究的大多数其他鳞翅目物种相比，这些 STR 检测到了非常高的遗传变异（平均 13.6 个等位基因，He = 0.75）。尽管可以检测到两个非空间聚类，但测试种群的空间遗传分化程度较低，基因流动水平较高。我们得出结论，这套遗传标记可能是监测该经济重要昆虫种群遗传多样性的有用资源。
