Toh Yi-Chin, Raja Anju, Yu Hanry, van Noort Danny
Department of Biomedical Engineering, 4 Engineering Drive, National University of Singapore, Singapore 117853, Singapore.
Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, #04-01, 31 Biopolis Way, Singapore 138669, Singapore.
Bioengineering (Basel). 2018 Apr 8;5(2):29. doi: 10.3390/bioengineering5020029.
We have developed a microfluidic-based culture chip to simulate cancer cell migration and invasion across the basement membrane. In this microfluidic chip, a 3D microenvironment is engineered to culture metastatic breast cancer cells (MX1) in a 3D tumor model. A chemo-attractant was incorporated to stimulate motility across the membrane. We validated the usefulness of the chip by tracking the motilities of the cancer cells in the system, showing them to be migrating or invading (akin to metastasis). It is shown that our system can monitor cell migration in real time, as compare to Boyden chambers, for example. Thus, the chip will be of interest to the drug-screening community as it can potentially be used to monitor the behavior of cancer cell motility, and, therefore, metastasis, in the presence of anti-cancer drugs.
我们开发了一种基于微流控的培养芯片，用于模拟癌细胞跨基底膜的迁移和侵袭。在这个微流控芯片中，构建了一个三维微环境，用于在三维肿瘤模型中培养转移性乳腺癌细胞（MX1）。加入了一种化学引诱剂以刺激细胞穿过膜的运动。我们通过追踪系统中癌细胞的运动来验证该芯片的实用性，结果表明它们在迁移或侵袭（类似于转移）。例如，与博伊登小室相比，我们的系统能够实时监测细胞迁移。因此，该芯片对于药物筛选领域将具有吸引力，因为它有可能用于监测抗癌药物存在时癌细胞的运动行为，进而监测转移情况。
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