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Pharmaceutics. 2011 Mar 11;3(1):107-24. doi: 10.3390/pharmaceutics3010107.
Cell migration and invasion are processes that offer rich targets for intervention in key physiologic and pathologic phenomena such as wound healing and cancer metastasis. With the advent of high-throughput and high content imaging systems, there has been a movement towards the use of physiologically relevant cell-based assays earlier in the testing paradigm. This allows more effective identification of lead compounds and recognition of undesirable effects sooner in the drug discovery screening process. This article will review the effective use of several principle formats for studying cell motility: scratch assays, transmembrane assays, microfluidic devices and cell exclusion zone assays.
细胞迁移和侵袭是在伤口愈合和癌症转移等关键生理和病理现象中进行干预的丰富目标。随着高通量和高内涵成像系统的出现，在测试范例中更早地使用基于生理相关细胞的测定法已经成为一种趋势。这使得在药物发现筛选过程中能够更有效地识别先导化合物并更早地发现不良影响。本文将综述几种研究细胞迁移的主要方法的有效应用：划痕实验、跨膜实验、微流控装置和细胞排除区实验。
