Tarekegn Getinet Mekuriaw, Ji Xiao-Yang, Bai Xue, Liu Bin, Zhang Wenguang, Birungi Josephine, Djikeng Appolinaire, Tesfaye Kassahun
Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala P.O. Box 7070, Sweden.
Department of Animal Production and Technology, Bahir Dar University, Bahir Dar P.O. Box 79, Ethiopia.
Asian-Australas J Anim Sci. 2018 Sep;31(9):1393-1400. doi: 10.5713/ajas.17.0596. Epub 2018 Apr 11.
This study was carried out to assess the haplotype diversity and population dynamics in cattle populations of Ethiopia.
We sequenced the complete mitochondrial cytochrome b gene of 76 animals from five indigenous and one Holstein Friesian×Barka cross bred cattle populations.
In the sequence analysis, 18 haplotypes were generated from 18 segregating sites and the average haplotype and nucleotide diversities were 0.7540±0.043 and 0.0010±0.000, respectively. The population differentiation analysis shows a weak population structure (4.55%) among the populations studied. Majority of the variation (95.45%) is observed by within populations. The overall average pair-wise distance (FST) was 0.049539 with the highest (FST = 0.1245) and the lowest (FST = 0.011) FST distances observed between Boran and Abigar, and Sheko and Abigar from the indigenous cattle, respectively. The phylogenetic network analysis revealed that all the haplotypes detected clustered together with the Bos taurus cattle and converged to a haplogroup. No haplotype in Ethiopian cattle was observed clustered with the reference Bos indicus group. The mismatch distribution analysis indicates a single population expansion event among the cattle populations.
Overall, high haplotype variability was observed among Ethiopian cattle populations and they share a common ancestor with Bos taurus.
开展本研究以评估埃塞俄比亚牛群的单倍型多样性和群体动态。
我们对来自五个本地牛群以及一个荷斯坦弗里生×巴尔卡杂交牛群的76头动物的线粒体细胞色素b基因进行了全序列测序。
在序列分析中，从18个分离位点产生了18种单倍型，平均单倍型多样性和核苷酸多样性分别为0.7540±0.043和0.0010±0.000。群体分化分析显示，在所研究的群体之间存在较弱的群体结构（4.55%）。大部分变异（95.45%）存在于群体内部。总体平均成对距离（FST）为0.049539，其中在本地牛群中，博拉牛和阿比加尔牛之间观察到的FST距离最高（FST = 0.1245），而谢科牛和阿比加尔牛之间观察到的FST距离最低（FST = 0.011）。系统发育网络分析表明，检测到的所有单倍型都与普通牛聚集在一起，并汇聚到一个单倍群。未观察到埃塞俄比亚牛的单倍型与参考瘤牛组聚集在一起。错配分布分析表明牛群中发生了一次群体扩张事件。
总体而言，埃塞俄比亚牛群中观察到较高的单倍型变异性，并且它们与普通牛拥有共同的祖先。
