[弱精子症男性精子中DKKL1的表达]
[Expression of DKKL1 in spermatozoa of men with asthenospermia].
作者信息
Yan Qiu-Xia, Ma Yi, Chen Run-Qiang, Zhou Xiu-Qin, Qiao Jing, Xian Ying-Jie, Feng Ling, Chen Cai-Rong
机构信息
1Center for Reproductive Medicine, Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan 511518,China; 2Department of Cellular Biology, Institute of Biological Medicine, Jinan University, Guangzhou 510632, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2018 Mar 20;38(3):324-328. doi: 10.3969/j.issn.1673-4254.2018.03.13.
OBJECTIVE
To compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia.
METHODS
The characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed using computer-assisted sperm analysis according to WHO criteria. The ejaculated sperms were isolated by Percoll discontinuous density gradients to detect the expression of DKKL1 mRNA and protein using real-time PCR and Western blotting.
RESULTS
The expression of DKKL1 mRNA was significantly down-regulated by 11.1 times in asthenospermic men as compared with that in normal fertile men (P<0.01). Western blotting showed that the expression of DKKL1 protein was down-regulated by 2.4 times in asthenospermic men compared to normal fertile men.
CONCLUSION
The expression of DKKL1, which may play an important role in sperm motility,is significantly decreased in ejaculated spermatozoa of men with asthenospermia.
目的
比较正常生育男性与弱精子症男性射出精子中DKKL1的表达情况，并探讨DKKL1在弱精子症发病机制中的作用。
方法
根据世界卫生组织标准，采用计算机辅助精子分析技术分析从正常生育男性和弱精子症男性收集的精液样本特征。通过Percoll不连续密度梯度分离射出的精子，采用实时PCR和蛋白质印迹法检测DKKL1 mRNA和蛋白质的表达。
结果
与正常生育男性相比，弱精子症男性中DKKL1 mRNA的表达显著下调11.1倍（P<0.01）。蛋白质印迹法显示，与正常生育男性相比，弱精子症男性中DKKL1蛋白的表达下调2.4倍。
结论
DKKL1的表达在精子活力中可能起重要作用，在弱精子症男性的射出精子中显著降低。
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