Hosseinzadeh Sevda, Rafat Seyed Abbas, Fang Lingzhao
Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Center for Quantitative Genetics and Genomics, Aarhus University, Aarhus, 8000, Denmark.
Sci Rep. 2025 Jan 14;15(1):1932. doi: 10.1038/s41598-024-82448-x.
Low fertility in cows leads to early removal from herds. Since reproductive traits are complex and have low heritability, genetic analysis can aid in improving reproduction. This study identified key genes linked to fertility by conducting genome- and transcriptome-wide association studies, RNA-seq analysis, meta-analysis, weighted gene co-expression network analysis, and functional enrichment analysis. Through these methods, we identified candidate genes related to Cow conception rate (CCR), Daughter pregnancy rate (DPR), Heifer conception rate (HCR), and overall fertility traits, helping to improve genetic selection for reproductive success in cows. The identified genes include RPL12, FKBP1B, FZD10, COX10, COX7A2, GAA, ETFBKMT, ACSM5, NUDT9, TIGAR, PAIP2, and PSMB5. Notably, GAA, ETFBKMT, COX10, and COX7A2 are involved in the "generation of precursor metabolites and energy" process. COX10, GAA, ETFBKMT, ACSM5, NUDT9, and TIGAR exhibit significant impacts on CCR, DPR, and HCR. COX7A2, PAIP2, and PSMB5 have been identified as hub genes related to fertility traits. RPL12 plays a role in protein synthesis, essential for gametogenesis and embryo development, while FKBP1B regulates calcium signaling, particularly in oocyte aging and fertility decline, and FZD10 is crucial in Wnt signaling. The identified genes serve as markers for genomic selection aimed at enhancing reproductive traits in cow.
奶牛的低繁殖力会导致其过早被淘汰出牛群。由于繁殖性状复杂且遗传力低，基因分析有助于改善繁殖性能。本研究通过进行全基因组和转录组关联研究、RNA测序分析、荟萃分析、加权基因共表达网络分析和功能富集分析，确定了与繁殖力相关的关键基因。通过这些方法，我们鉴定出了与奶牛受孕率（CCR）、女儿怀孕率（DPR）、小母牛受孕率（HCR）以及总体繁殖性状相关的候选基因，有助于改进奶牛繁殖成功的基因选择。鉴定出的基因包括RPL12、FKBP1B、FZD10、COX10、COX7A2、GAA、ETFBKMT、ACSM5、NUDT9、TIGAR、PAIP2和PSMB5。值得注意的是，GAA、ETFBKMT、COX10和COX7A2参与“前体代谢物和能量的生成”过程。COX10、GAA、ETFBKMT、ACSM5、NUDT9和TIGAR对CCR、DPR和HCR有显著影响。COX7A2、PAIP2和PSMB5已被确定为与繁殖性状相关的枢纽基因。RPL12在蛋白质合成中起作用，这对配子发生和胚胎发育至关重要，而FKBP1B调节钙信号，特别是在卵母细胞衰老和繁殖力下降方面，FZD10在Wnt信号传导中起关键作用。鉴定出的这些基因可作为基因组选择的标记，旨在增强奶牛的繁殖性状。
