一种基于抗坏血酸氧化酶的传感平台，用于与抗坏血酸和异抗坏血酸进行立体选择性相互作用。

An Ascorbic Acid Oxidase-based Sensing Platform for Stereoselective Interaction with Ascorbic Acid and Isoascorbic Acid.

作者信息

Ma Jiao, Xu Juanjuan, Yang Chengcheng, Song Jinyi, Fu Yingzi

机构信息

Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University.

Mianyang Product Quality Supervision & Inspection Institute.

出版信息

Anal Sci. 2018;34(4):427-432. doi: 10.2116/analsci.17P304.

DOI:10.2116/analsci.17P304

PMID:29643305

Abstract

A simple enzyme-based nanohybrid material was fabricated via immobilizing ascorbic acid oxidase (AO) on the surface of flower-like electrodeposited gold nanoparticles (dpAu) and reduced graphene oxide (rGO) modified glassy carbon electrodes (GCEs). The composite material was used for stereoselective interaction with ascorbic acid (AA) and isoascorbic acid (IAA). Herein, AO was applied as a stereoselective selector, and the dpAu/rGO nanohybrid not only acted as a supporter for high loading of AO, but also served as the nanomaterial for signal amplification. The results showed obvious peak current differences between AA and IAA, indicating that this strategy could be employed to recognize AA and IAA. Under the optimum conditions, the sensor exhibited a good linear response to AA and IAA in a linear range of 1.0 × 10 - 5.0 × 10 M. This approach with the merits of simplicity and rapid response provided a promising perspective for identification of AA and IAA.

摘要

通过将抗坏血酸氧化酶（AO）固定在花状电沉积金纳米颗粒（dpAu）和还原氧化石墨烯（rGO）修饰的玻碳电极（GCE）表面，制备了一种基于简单酶的纳米杂化材料。该复合材料用于与抗坏血酸（AA）和异抗坏血酸（IAA）进行立体选择性相互作用。在此，AO用作立体选择性选择剂，dpAu/rGO纳米杂化物不仅作为高负载AO的载体，还作为信号放大的纳米材料。结果显示AA和IAA之间存在明显的峰值电流差异，表明该策略可用于识别AA和IAA。在最佳条件下，该传感器对AA和IAA在1.0×10 - 5.0×10 M的线性范围内表现出良好的线性响应。这种方法具有简单和响应快速的优点，为AA和IAA的识别提供了一个有前景的前景。