Pumeesat Potjaman, Wongsuk Thanwa, Muangkaew Watcharamat, Luplertlop Natthanej
Southeast Asian J Trop Med Public Health. 2017 Jan;48(1):170-8.
Scedosporium boydii and Lomentospora prolificans are filamentous fungi reported to cause infection in immunocompromized individuals. We studied the effect of farnesol to inhibit growth of S. boydii and L. prolificans by measuring colony diameter and determining minimal effective concentration (MEC). S. boydii and L. prolificans were grown on Sabouraud dextrose agar (SDA) at 37oC for 5 days. Conidia were collected and adjusted to a concentration of 104 conidia/ ml. Twenty microliters of conidia suspension was placed in each well of a sixwell plate containing serial dilutions of farnesol (10 μM, 100 μM, 1,000 μM, and 10,000 μM) in SDA. Colony morphology and diameter were observed on days 1, 2, 3, and 4. Farnesol at concentrations of 1,000 μM or higher caused the colony diameter of both S. boydii and L. prolificans to be smaller than untreated controls in a dose-dependent manner. The MEC of farnesol to inhibit growth of both S. boydii and L. prolificans was 3.2 mM. This study reveals the antifungal property of farnesol against S. boydii and L. prolificans, which can be used for further study as an alternative antifungal agent against these fungal infections.
博伊德氏足分支霉和多育镰刀菌是据报道可在免疫功能低下个体中引起感染的丝状真菌。我们通过测量菌落直径和确定最小有效浓度（MEC）来研究法尼醇抑制博伊德氏足分支霉和多育镰刀菌生长的效果。将博伊德氏足分支霉和多育镰刀菌接种于沙氏葡萄糖琼脂（SDA）上，在37℃培养5天。收集分生孢子并将其浓度调整至10⁴个分生孢子/毫升。将20微升分生孢子悬液加入到含有不同系列稀释浓度法尼醇（10μM、100μM、1000μM和10000μM）的SDA的六孔板的每个孔中。在第1、2、3和4天观察菌落形态和直径。浓度为1000μM或更高的法尼醇可使博伊德氏足分支霉和多育镰刀菌的菌落直径以剂量依赖方式小于未处理的对照。法尼醇抑制博伊德氏足分支霉和多育镰刀菌生长的MEC为3.2mM。本研究揭示了法尼醇对博伊德氏足分支霉和多育镰刀菌的抗真菌特性，其可作为抗这些真菌感染的替代抗真菌剂用于进一步研究。
