Ko Kyung-Seok, Kong In Chul
J Nanosci Nanotechnol. 2017 Apr;17(4):2382-389. doi: 10.1166/jnn.2017.13098.
The effects of the modified incubation conditions of a conventional bioassay on the toxicity of partially soluble nanoparticles (NPs) were evaluated based on the activity of seed germination and bacterial bioluminescence. Different levels of toxicity were observed for seed germination (CuO > ZnO > NiO) and bacterial bioluminescence (ZnO > CuO > NiO). The NP inhibition of seed germination increased strongly under modified incubation conditions: sample volume from 5 mL to 10 mL, shaking from none to 70 rpm, and working vessel from a Petri dish (+/− filter paper) to an Erlenmeyer flask (no filter paper). In the case of seed germination, the toxicity levels of NPs under the modified conditions were 1.26 to 8.49 times higher than the conventional method according to the type of NPs and modified conditions (p-values < 0.05). No significant differences in bacterial bioluminescence were observed between conventional (130 rpm) and modified (160 rpm) conditions. These findings show that for an accurate assessment of partially soluble NPs toxicity in ecosystems, the conventional bioassay method, which is designed for soluble chemicals, needs to be performed under modified conditions because of their insolubility.
基于种子发芽活性和细菌生物发光，评估了常规生物测定中改良培养条件对部分可溶性纳米颗粒（NPs）毒性的影响。观察到种子发芽（CuO>ZnO>NiO）和细菌生物发光（ZnO>CuO>NiO）的不同毒性水平。在改良培养条件下，NPs对种子发芽的抑制作用显著增强：样品体积从5 mL增加到10 mL，振荡从无到70 rpm，工作容器从培养皿（±滤纸）变为锥形瓶（无滤纸）。就种子发芽而言，根据NPs类型和改良条件，改良条件下NPs的毒性水平比传统方法高1.26至8.49倍（p值<0.05）。在传统条件（130 rpm）和改良条件（160 rpm）之间，未观察到细菌生物发光的显著差异。这些发现表明，为了准确评估部分可溶性NPs在生态系统中的毒性，由于其不溶性，针对可溶性化学物质设计的传统生物测定方法需要在改良条件下进行。
