Khan Abdul Qayum, Ahmad Taseer, Mushtaq Muhammad Naveed, Malik Muhammad Nasir Hayat, Naz Huma, Ahsan Haseb, Asif Hira, Noor Nabeela, Rahman Muhammad Shafiq Ur, Dar Umair, Rashid Muhammad
Acta Pol Pharm. 2016 Jul;73(4):949-954.
Ranunculus muricatus Linn. (RML) have been traditionally used for the treatment of various cardiovascular disorders. The aim of present study was to evaluate their cardiovascular effects in isolated perfused rabbit heart. The methanolic extract of RML was prepared by cold maceration process. The methanolic extract of RML (1 ng to 10 mg) was used to determine the percentage change in force of contraction (FC), heart rate (HR) and perfusion pressure (PP) by using Langendorff's Perfused Heart Apparatus. The PP, FC and HR of isolated rabbit heart were measured by power lab data acquisition system. Moreover, phytochemical analysis and acute toxicity study were also performed. The methanolic extract at the doses from I ng to 10 mg exhibited a significant increase in perfusion pressure and force of contraction. Moreover, the crude extract of RML revealed a significant increase in heart rate at doses from 1 ng to μg. The maximum rise in all the thee parameters was observed at 1 μg and 1 ng, respectively In another study, the melhanoliC extract was tested in the presence of propranolol and verapamil on isolated perfused rabbit heart. The study shown that the increase in HR and FC produced by the plant extracts was significantly reduced in the presence of propranolol whereas PP remained significantly raised even in the presence of propranolol. However, in the presence of verapamil, this increased PP was significantly reversed to a decrease while a significant positive inotropic and chronotropic effects were observed. It is concluded that the cardiotonic activity of methanolic extract of RML might be due certain cardio active chemical compounds. Further studies are needed to isolate these pharmacologically active phytochemical constituents and elucidate their exact mechanism of action.
毛茛（Ranunculus muricatus Linn.，RML）传统上用于治疗各种心血管疾病。本研究的目的是评估其对离体灌注兔心脏的心血管作用。RML的甲醇提取物通过冷浸法制备。使用Langendorff离体心脏灌注装置，用RML的甲醇提取物（1纳克至10毫克）来测定收缩力（FC）、心率（HR）和灌注压（PP）的变化百分比。离体兔心脏的PP、FC和HR通过PowerLab数据采集系统进行测量。此外，还进行了植物化学分析和急性毒性研究。剂量为1纳克至10毫克的甲醇提取物使灌注压和收缩力显著增加。此外，RML的粗提取物在剂量为1纳克至微克时使心率显著增加。在1微克和1纳克时分别观察到所有这三个参数的最大升高。在另一项研究中，在普萘洛尔和维拉帕米存在的情况下，对离体灌注兔心脏进行了甲醇提取物测试。研究表明，在普萘洛尔存在的情况下，植物提取物引起的HR和FC增加显著降低，而即使在普萘洛尔存在的情况下，PP仍显著升高。然而，在维拉帕米存在的情况下，这种升高的PP显著逆转至降低，同时观察到显著的正性肌力和变时作用。得出的结论是，RML甲醇提取物的强心活性可能归因于某些具有心脏活性的化合物。需要进一步研究来分离这些具有药理活性的植物化学成分并阐明其确切作用机制。
