Department of Anesthesiology and Pain Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
PLoS One. 2018 Apr 12;13(4):e0195262. doi: 10.1371/journal.pone.0195262. eCollection 2018.
Liver graft regeneration is orchestrated by specific and sequential stimuli, including hepatocyte growth factors, cytokines, and catecholamines. We evaluated the association between preoperative serum cytokines and early liver graft regeneration in human living donor liver transplantation (LDLT).
We retrospectively reviewed the data of adult patients who underwent LDLT from January 2010 to December 2014. Serum cytokines, including interleukin (IL)-2, 6, 10, 12, 17, interferon (IFN)-γ and tumor necrosis factor (TNF)-α were measured in the recipients 1 day before surgery and on postoperative day (POD) 7. Liver graft volume was estimated using abdominal computed tomography images of the donors and recipients.
In total, 226 patients were analyzed in this study. Median preoperative levels of serum cytokines were as follows: IL-2, 0.1 (0.1-1.6) pg/mL; IL-6, 7.3 (0.1-30.2) pg/mL; IL-10, 0.5 (0.1-11.0) pg/mL; IL-12, 0.1 (0.1-0.1) pg/mL; IL-17, 2.0 (0.1-16.4) pg/mL; IFN-γ, 3.2 (0.1-16.0) pg/mL; and TNF-α, 9.8 (5.4-17.9) pg/mL. Higher preoperative serum levels of IL-6, IL-10, and TNF-α, dichotomized at the median, were associated with increased relative liver volumes by POD 7. Multivariate analysis revealed that higher levels of serum IL-6 and TNF-α were independently associated with increased graft volume during the first 1 week after LDLT, based on the lower levels of those cytokines.
IL-6 and TNF-α were important mediators of the success of early graft regeneration in patients who underwent LDLT.
肝移植物再生是由特定的、序贯的刺激物调控的，包括肝细胞生长因子、细胞因子和儿茶酚胺。我们评估了术前血清细胞因子与人类活体肝移植（LDLT）中早期肝移植物再生之间的关系。
我们回顾性分析了 2010 年 1 月至 2014 年 12 月期间接受 LDLT 的成年患者的数据。在手术前 1 天和术后第 7 天（POD），受体的血清细胞因子（白细胞介素（IL）-2、6、10、12、17、干扰素（IFN）-γ和肿瘤坏死因子（TNF）-α）被测量。使用供体和受体的腹部计算机断层扫描图像来估计肝移植物的体积。
本研究共分析了 226 例患者。术前血清细胞因子中位数如下：IL-2，0.1（0.1-1.6）pg/mL；IL-6，7.3（0.1-30.2）pg/mL；IL-10，0.5（0.1-11.0）pg/mL；IL-12，0.1（0.1-0.1）pg/mL；IL-17，2.0（0.1-16.4）pg/mL；IFN-γ，3.2（0.1-16.0）pg/mL；和 TNF-α，9.8（5.4-17.9）pg/mL。术前血清 IL-6、IL-10 和 TNF-α的水平较高，中位数为 7.3pg/mL，与术后第 7 天相对肝体积增加有关。多变量分析显示，基于较低的细胞因子水平，术前血清 IL-6 和 TNF-α水平较高与 LDLT 后第一周肝移植物体积增加独立相关。
IL-6 和 TNF-α是 LDLT 患者早期移植物再生成功的重要介质。
