A rapid and sensitive enzyme immunoassay for Cu/Zn superoxide dismutase with polyclonal and monoclonal antibodies.
作者信息
Porstmann T, Wietschke R, Schmechta H, Grunow R, Porstmann B, Bleiber R, Pergande M, Stachat S, von Baehr R
机构信息
Institute of Medical Immunology, Faculty of Medicine, Humboldt University of Berlin, GDR.
出版信息
Clin Chim Acta. 1988 Jan 15;171(1):1-10. doi: 10.1016/0009-8981(88)90285-9.
An enzyme immunoassay for the quantification of human Cu/Zn SOD in serum, urine and erythrocytes was developed applying monoclonal and polyclonal antibodies. The one-step assay is completed within 30 min and enables the detection of 0.3 microgram Cu/Zn SOD per litre. A Cu/Zn SOD concentration of 46 +/- 21.5 micrograms/l and of 1 +/- 0.6 micrograms/mmol creatinine was determined in the serum and the urine, respectively, of healthy individuals. A content of 15 +/- 1.7 ng Cu/Zn SOD was found in 10(6) erythrocytes. Patients with Down's syndrome exhibited a 3.8-fold, a 2-fold and a 1.6-fold higher concentration of Cu/Zn SOD in their serum, urine and erythrocytes.
Zotero 插件