人铜锌超氧化物歧化酶的放射免疫测定法及其在红细胞中的应用。

A radioimmune assay for human cupro-zinc superoxide dismutase and its application to erythrocytes.

作者信息

Del Villano B C, Tischfield J A

出版信息

J Immunol Methods. 1979;29(3):253-62. doi: 10.1016/0022-1759(79)90313-2.

Abstract

A competition radioimmunoassay for human superoxide dismutase (SOD-1) is described. Radioiodinated SOD-1 is incubated with unlabeled competitor and limiting antibody, then immune complexes are isolated using Staphylococcus aureus. The assay is sensitive (detecting 5 ng enzyme) and reproducible. Average values for SOD-1 in lysates of erythrocytes from normal individuals is 854 +/- 100 ng/mg hemoglobin and that of patients with trisomy-21 is 1313 +/- 110 ng/mg hemoglobin.

摘要

本文描述了一种用于检测人超氧化物歧化酶（SOD-1）的竞争性放射免疫测定法。将放射性碘化的SOD-1与未标记的竞争物和限量抗体一起孵育，然后使用金黄色葡萄球菌分离免疫复合物。该测定法灵敏度高（可检测5纳克酶）且可重复。正常个体红细胞裂解物中SOD-1的平均值为854±100纳克/毫克血红蛋白，21三体综合征患者的该平均值为1313±110纳克/毫克血红蛋白。