Universidade Federal de Viçosa, Departamento de Veterinária, Campus UFV, Centro, 36570 900 Viçosa, MG, Brazil.
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Av. do Café S/N, sala 41, Bloco S, 14040-903 Ribeirão Preto, SP, Brazil.
Int J Food Microbiol. 2018 Jul 2;276:5-9. doi: 10.1016/j.ijfoodmicro.2018.03.028. Epub 2018 Apr 3.
This study aimed to track Yersinia enterocolitica contamination in a pork production chain in Minas Gerais, Brazil, and to characterize the virulence and antibiotic resistance of isolates. Samples were collected from four different steps of the pork production chain (pig farm, carcass, processing environment and end product; n = 870), and tested for the presence of Y. enterocolitica. The pathogen was detected in 8 samples (palatine tonsils = 5; mesenteric lymph nodes = 2; carcass after bleeding = 1), from which 16 isolates were obtained and identified as Y. enterocolitica bioserotype 4/O:3. XbaI macrorestriction allowed the clustering of isolates in 5 pulsetypes, and the identification of identical profiles of Y. enterocolitca isolated from different samples. All isolates were positive for the virulence related genes ail, virF, myfA, ystA, tccC, ymoA, hreP and sat, and negative for ystB, ystC, fepA, fepD and fes. Considering 17 antibiotics from 11 classes, only ciprofloxacin and kanamycin were effective against all isolates, and three multidrug resistance profiles were identified among them, with simultaneous resistance to 9 of 11 classes. All isolates presented positive results for emrD, yfhD and marC, related to multidrug resistance. The results of this study demonstrated the contamination routes of Y. enterocolitica within the assessed pork production chain, and highlighted the pathogenic potential and antibiotic resistance of this foodborne pathogen.
本研究旨在追踪巴西米纳斯吉拉斯州猪肉生产链中的耶尔森氏菌污染情况，并对分离株的毒力和抗生素耐药性进行特征分析。从猪肉生产链的四个不同步骤（猪场、胴体、加工环境和最终产品；n=870）采集样本，并检测是否存在耶尔森氏菌。在 8 个样本（扁桃体=5；肠系膜淋巴结=2；放血后胴体=1）中检测到该病原体，从中获得 16 个分离株，并鉴定为 4/O:3 型肠炎耶尔森氏菌。XbaI 宏限制允许将分离株聚类为 5 个脉冲型，并鉴定出从不同样本分离的相同的耶尔森氏菌 profiles。所有分离株均携带与毒力相关的基因 ail、virF、myfA、ystA、tccC、ymoA、hreP 和 sat，不携带 ystB、ystC、fepA、fepD 和 fes。考虑到来自 11 个类别的 17 种抗生素，只有环丙沙星和卡那霉素对所有分离株有效，并且在其中鉴定出三种多药耐药 profile，同时对 11 个类别的 9 种抗生素耐药。所有分离株均对 emrD、yfhD 和 marC 呈阳性，这与多药耐药性有关。本研究结果表明了在评估的猪肉生产链中耶尔森氏菌的污染途径，并强调了这种食源性病原体的致病性和抗生素耐药性。
