Department of Chemistry , Southern University of Science and Technology , 518055 Shenzhen , China.
Anal Chem. 2018 May 1;90(9):5818-5824. doi: 10.1021/acs.analchem.8b00360. Epub 2018 Apr 19.
Nanoscale ionophore-based ion-selective optodes (nano-ISOs) are effective sensing tools for in situ and real time measurements of ion concentrations in biological and environmental samples. While searching for novel sensing materials, nano-ISOs free of plasticizers are particularly important for biological and environmental applications. This work described plasticizer-free nano-ISOs based on Si-containing particles including PEGylated organosilica nanoparticles, PDMS nanospheres, and SiO microspheres, with diameters around 50 nm, 100 nm, and 5 μm, respectively. The platform enabled the use of highly selective ionophores, where the nanomatrices played important roles in tuning the ion-carrier complex formation constants and led to better selectivity for the PEGylated organosilica nano-ISOs than those based on PDMS. With use of the versatile silica chemistry, pH and ion dual sensing was achieved on SiO microspheres. In addition, increasing the cross-linking degree of the PDMS nano-ISOs extended the linear response range, and cellular uptake experiments showed that the nano-ISOs could readily enter HeLa cells with very low cytotoxicity.
基于纳米离子载体的离子选择性光导纤维（nano-ISOs）是用于原位和实时测量生物和环境样品中离子浓度的有效传感工具。在寻找新型传感材料时，不含增塑剂的 nano-ISOs 对于生物和环境应用尤为重要。本工作描述了基于含硅颗粒的无增塑剂 nano-ISOs，包括聚乙二醇化有机硅纳米粒子、PDMS 纳米球和 SiO 微球，其直径分别约为 50nm、100nm 和 5μm。该平台能够使用高选择性离子载体，其中纳米基质在调节离子载体配合物形成常数方面发挥着重要作用，导致基于聚乙二醇化有机硅纳米粒子的 nano-ISOs 具有比基于 PDMS 的 nano-ISOs 更好的选择性。利用多功能的硅化学，在 SiO 微球上实现了 pH 和离子双重传感。此外，增加 PDMS 纳米光导纤维的交联度扩展了线性响应范围，细胞摄取实验表明，纳米光导纤维很容易进入 HeLa 细胞，且细胞毒性非常低。
