Du Xinfeng, Li Niping, Chen Qinghan, Wu Zeying, Zhai Jingying, Xie Xiaojiang
School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China.
Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.
Biomicrofluidics. 2022 Jun 10;16(3):031301. doi: 10.1063/5.0090599. eCollection 2022 May.
Inorganic ions are ubiquitous in all kinds of cells with highly dynamic spatial and temporal distribution. Taking advantage of different types of fluorescent probes, fluorescence microscopic imaging and quantitative analysis of ion concentrations in cells have rapidly advanced. A family of fluorescent nanoprobes based on ionophores has emerged in recent years with the potential to establish a unique platform for the analysis of common biological ions including Na, K, Ca, Cl, and so on. This article aims at providing a retrospect and outlook of ionophore-based ion-selective nanoprobes and the applications in cell imaging.
无机离子普遍存在于各类细胞中，具有高度动态的时空分布。借助不同类型的荧光探针，细胞内离子浓度的荧光显微成像及定量分析取得了快速进展。近年来，基于离子载体的荧光纳米探针家族应运而生，有潜力建立一个独特的平台，用于分析包括钠、钾、钙、氯等在内的常见生物离子。本文旨在对基于离子载体的离子选择性纳米探针及其在细胞成像中的应用进行回顾与展望。
