Mori Miho, Sakagami Yoshikazu, Hamazaki Yousuke, Jojima Toru
Faculty of Agriculture, Kindai University , Nara City , Japan.
Environ Technol. 2019 Sep;40(23):3094-3104. doi: 10.1080/09593330.2018.1465128. Epub 2018 Apr 23.
When infectious diseases arise in domestic animals, a large amount of slaked lime is sprinkled on cattle sheds and their surroundings for disinfection and prevention. However, optimal sprinkling methods, standard and upper limit of slaked lime, and influence of slaked lime on non-target microorganisms remain unclear. In this study, we clarified detailed microbicidal effects of slaked lime via experiments and the influence of sprinkling powdered slaked lime (PSL) in field soil on microorganisms. disinfection tests assessing the appropriate amount of water and ventilation conditions were also performed in sterilized glass bottles with soil and subsp. serovar Typhimurium. Under conditions with a small amount of water relative to the amount of PSL, the bactericidal effect and sustainability of powdered slaked lime (PSL) tended to be lower than those without spraying water. Moreover, the sterilization effect markedly decreased after 7 days under conditions with abundant water. These results indicate that the amount of sprayed water is very important for the bactericidal effect and persistence of PSL. A field experiment showed that the pH and exchange calcium (Ca) content of the soil sprinkled with over 1000 g m PSL remained high even after a long period (≥1 year), with values of approximately 0.5-1.0 and approximately 3-11 times the level without PSL, respectively. However, sprinkling PSL did not influence viable microbial counts at any concentration.
当家畜发生传染病时，会在牛舍及其周围撒大量熟石灰进行消毒和预防。然而，熟石灰的最佳撒施方法、标准和上限以及熟石灰对非目标微生物的影响仍不清楚。在本研究中，我们通过实验阐明了熟石灰的详细杀菌效果以及在田间土壤中撒施熟石灰粉（PSL）对微生物的影响。还在装有土壤和鼠伤寒沙门氏菌亚种血清型的无菌玻璃瓶中进行了评估适量水分和通风条件的消毒试验。在相对于PSL量水分较少的条件下，熟石灰粉（PSL）的杀菌效果和持续性往往低于未喷水的情况。此外，在水分充足的条件下，7天后杀菌效果明显下降。这些结果表明，喷水量对PSL的杀菌效果和持久性非常重要。一项田间试验表明，即使经过很长一段时间（≥1年），每平方米撒施超过1000克PSL的土壤的pH值和交换性钙（Ca）含量仍然很高，分别约为未撒施PSL土壤的0.5 - 1.0倍和约3 - 11倍。然而，无论浓度如何，撒施PSL均不影响活菌数。
