棉酚对分离线粒体的生化作用：单价阳离子与ATP酶活性

Biochemical effects of gossypol in isolated mitochondria: monovalent cations and ATPase activity.

Martínez F, Gamboa S, Díaz-Sánchez V

Departamento de Bioquímica, Facultad de Medicina, México, D.F. México.

Int J Biochem. 1988;20(2):189-92. doi: 10.1016/0020-711x(88)90485-5.

Abstract

The effect of gossypol acetic acid upon the energy-producing system of isolated liver mitochondria was studied. Gossypol was found to exert an uncoupling effect. 2. Oxygen consumption and ATPase activity were stimulated only when a monovalent alkali metal cations was present. 3. The mitochondrial proton gradient, produced by the enzymatic hydrolysis of ATP, was lower in the gossypol-treated mitochondria than in the control experiments. 4. It is proposed that gossypol stimulates oxygen consumption and the ATPase activity in mitochondria by a protonophoretic effect and a cation translocation process.

摘要

研究了醋酸棉酚对分离的肝线粒体能量产生系统的作用。发现棉酚具有解偶联作用。2. 仅当存在一价碱金属阳离子时，氧消耗和ATP酶活性才会受到刺激。3. 经棉酚处理的线粒体中，由ATP酶促水解产生的线粒体质子梯度低于对照实验中的质子梯度。4. 有人提出，棉酚通过质子载体效应和阳离子转运过程刺激线粒体中的氧消耗和ATP酶活性。