Yeoman H, Robins R A
Cancer Research Campaign Laboratories, University Park, Nottingham, U.K.
Immunology. 1988 Feb;63(2):291-7.
The effect of exposing tumour cells to interferon-gamma (IFN-gamma) has been studied investigating changes in MHC antigen expression and susceptibility to a variety of cellular effector mechanisms. Treatment of rat tumour cell lines with rat recombinant IFN-gamma increased their expression of class I MHC molecules, as monitored by quantitative immunofluorescence. Reduced sensitivity to lysis by NK cells was observed, although cold-target competition assays indicated NK cells were bound equally well by both interferon-treated and control cells. Decreased susceptibility to NK lysis was not a general effect of IFN-gamma treatment of tumour cells, as the sensitivity to lysis by activated macrophages was unaffected. Furthermore, both allogeneic CTL and CTL from syngeneic tumour-immune rats were able to kill IFN-gamma-treated tumour cells more effectively than untreated control target cells. These studies show that IFN changes the balance of sensitivity to different cytolytic effector mechanisms, increasing T-cell mediated effects, decreasing NK killing, but leaving macrophage cytotoxicity unaffected. The impact of these changes on tumour rejection will depend on the relative contribution of these mechanisms to target-cell destruction.
研究了将肿瘤细胞暴露于γ干扰素（IFN-γ）的效果，观察主要组织相容性复合体（MHC）抗原表达的变化以及对多种细胞效应机制敏感性的变化。用大鼠重组IFN-γ处理大鼠肿瘤细胞系后，通过定量免疫荧光监测发现，I类MHC分子的表达增加。虽然冷靶竞争试验表明NK细胞与经干扰素处理的细胞和对照细胞的结合情况相同，但观察到经IFN-γ处理的肿瘤细胞对NK细胞裂解的敏感性降低。对肿瘤细胞进行IFN-γ处理并不会普遍降低其对NK裂解的敏感性，因为对活化巨噬细胞裂解的敏感性并未受到影响。此外，来自同种异体的细胞毒性T淋巴细胞（CTL）以及来自同基因肿瘤免疫大鼠的CTL，都能够比未处理的对照靶细胞更有效地杀伤经IFN-γ处理的肿瘤细胞。这些研究表明，IFN改变了对不同溶细胞效应机制的敏感性平衡，增强了T细胞介导的效应，降低了NK杀伤作用，但未影响巨噬细胞的细胞毒性。这些变化对肿瘤排斥反应的影响将取决于这些机制对靶细胞破坏的相对贡献。
