Bourret R B, Fox M S
Massachusetts Institute of Technology, Cambridge 02139.
J Bacteriol. 1988 Apr;170(4):1683-90. doi: 10.1128/jb.170.4.1683-1690.1988.
Characterization of a putative intermediate in the Mu lysogenization pathway is possible in a variant Escherichia coli himD strain which exhibits greatly diminished lysogen formation. In this strain, most infecting Mu genomes form stable, transcribable, nonreplicating structures. Many of these genomes can be mobilized to form lysogens by a second Mu infection, which can be delayed by at least 100 min. This intermediate structure can be formed in the absence of Mu A or B function. We suggest that the inferred intermediate could be the previously reported protein-linked circular form of the Mu genome. Providing Mu B function from a plasmid enhances Mu lysogenization in this him strain, and the enhancement is much greater when both Mu A and B functions are provided.
在一种溶源形成显著减少的大肠杆菌himD变异菌株中，可以对Mu溶源化途径中的假定中间体进行表征。在该菌株中，大多数感染的Mu基因组形成稳定、可转录、非复制性结构。这些基因组中的许多可以通过第二次Mu感染被动员形成溶源菌，第二次感染可延迟至少100分钟。这种中间结构可以在没有Mu A或B功能的情况下形成。我们认为推断出的中间体可能是先前报道的Mu基因组的蛋白质连接环状形式。从质粒提供Mu B功能可增强该him菌株中的Mu溶源化，当同时提供Mu A和B功能时，增强作用更大。
