噬菌体Mu的gemts突变体在大肠杆菌K12中诱导的同步分裂。

Synchronous division induced in Escherichia coli K12 by gemts mutants of phage Mu.

作者信息

Paolozzi L, Nicosia A, Liebart J C, Ghelardini P

机构信息

Centro studi per gli Acidi Nucleici del C.N.R., Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, Italy.

出版信息

Mol Gen Genet. 1989 Jul;218(1):13-7. doi: 10.1007/BF00330559.

DOI:10.1007/BF00330559

PMID:2528678

Abstract

Infection with the bacteriophage mutant Mu c+ gemts2 at 42 degrees C induces synchrony in cell division in cultures of Escherichia coli K12. This synchrony may last for several cycles and is not only due to selection since synchronization is observed even when bacterial survival to the infection is over 80% as in lysogens for Mu c+ gemts2. The mechanism by which synchrony is induced is not known, but since the product of Mu gene gem (previously called lig) has been shown to interact with the enzymatic system in the bacteria controlling the degree of DNA supercoiling, the phenomenon could be a consequence of this interaction.

摘要

在42摄氏度下用噬菌体突变体Mu c+ gemts2感染，可诱导大肠杆菌K12培养物中的细胞分裂同步化。这种同步化可能持续几个周期，且不仅仅是由于选择作用，因为即使在细菌对感染的存活率超过80%时（如Mu c+ gemts2的溶源菌）也能观察到同步化。诱导同步化的机制尚不清楚，但由于已表明Mu基因gem（以前称为lig）的产物可与细菌中控制DNA超螺旋程度的酶系统相互作用，所以这种现象可能是这种相互作用的结果。