Amadieu M, Giry J, Barlet J P
U.A. C.N.R.S. 1123, Ceyrat, France.
J Dev Physiol. 1988 Feb;10(1):37-45.
The effects of synthetic Atrial Natriuretic Factor (ANF) on urine flow rate, sodium excretion, potassium excretion and arterial blood pressure were studied in 10-12 days-old female calves. In four female calves fitted with a Foley catheter, an intravenous administration of ANF (Ile-ANF 26; 1.6 micrograms/kg body wt during 30 min) induced an increase (P less than 0.01) in urine flow rate (from 1.8 +/- 0.2 to 12.8 +/- 1.1 ml/min), sodium excretion (from 0.15 +/- 0.02 to 0.81 +/- 0.06 mmol/min) and free water clearance (from 0.13 +/- 0.9 to 5.16 +/- 0.5 ml/min). It had no significant effect on potassium excretion. In four calves chronically-instrumented with a carotid catheter, an intravenous administration of synthetic ANF alone (1.6 micrograms/kg body wt during 30 min) induced a gradual decrease (P less than 0.01) in systolic, diastolic and mean arterial blood pressure (from 112 +/- 4 to 72, from 72 +/- 2 to 61 +/- 1 and from 90 +/- 2 to 65 +/- 2 mmHg respectively, at the end of ANF infusion). An intravenous administration of angiotensin II (AII) (0.5 micrograms/kg body wt during 45 min) induced a significant increase in systolic, diastolic and mean arterial blood pressure which was antagonized by an i.v. bolus injection of ANF (0.125 micrograms/kg body wt). However, during a simultaneous administration of AII (0.3 micrograms/kg body wt during 30 min) and ANF (1.6 micrograms/kg body wt. during 30 min), the atrial peptide did not influence the pressure action of AII. These findings indicate that the conscious newborn calf is sensitive to diuretic, natriuretic and hypotensive effects of synthetic ANF.
研究了合成心房利钠因子（ANF）对10 - 12日龄雌性犊牛尿流率、钠排泄、钾排泄和动脉血压的影响。在4头安装了Foley导管的雌性犊牛中，静脉注射ANF（Ile - ANF 26；30分钟内1.6微克/千克体重）可使尿流率（从1.8±0.2增加到12.8±1.1毫升/分钟）、钠排泄（从0.15±0.02增加到0.81±0.06毫摩尔/分钟）和自由水清除率（从0.13±0.9增加到5.16±0.5毫升/分钟）显著增加（P＜0.01）。对钾排泄无显著影响。在4头长期安装颈动脉导管的犊牛中，单独静脉注射合成ANF（30分钟内1.6微克/千克体重）可使收缩压、舒张压和平均动脉血压逐渐降低（P＜0.01）（在ANF输注结束时，分别从112±4降至72、从72±2降至61±1和从90±2降至65±2毫米汞柱）。静脉注射血管紧张素II（AII）（45分钟内0.5微克/千克体重）可使收缩压、舒张压和平均动脉血压显著升高，而静脉推注ANF（0.125微克/千克体重）可拮抗这种升高。然而，在同时给予AII（30分钟内0.3微克/千克体重）和ANF（30分钟内1.6微克/千克体重）时，心房肽不影响AII的升压作用。这些发现表明，清醒的新生犊牛对合成ANF的利尿、利钠和降压作用敏感。
