静脉注射α-人心房钠尿肽（99-126）对清醒小牛血流动力学和肾脏影响的分离
Dissociation of hemodynamic and renal effects of i.v. alpha-hANF (99-126) in conscious calves.
作者信息
Windberger U, Vierhapper H, Osterode W, Nowotny P, Losert U
机构信息
Zentrum für Biomedizinische Forschung, Universität Wien, Austria.
出版信息
Horm Metab Res. 1993 May;25(5):259-63. doi: 10.1055/s-2007-1002092.
The effects of i.v. human atrial natriuretic factor (alpha-hANF [99-126]) was investigated in 5 conscious calves (age 4 months, weight 94 +/- 14kg) with constant fluid (300 ml/h) and sodium intake over a period of 24 hours before and during the 3 hours of experimentation. We administered 200 micrograms, 400 micrograms and 800 micrograms ANF at hourly intervals. Immediately (+2 min) after the i.v. injection the peptide's serum-concentration rose from, basal 13 +/- 3 to 802 +/- 191 (200 micrograms), 1707 +/- 419 (400 micrograms) and 3483 +/- 878 pmol/l (800 micrograms) (p < 0.0001), respectively. Mean arterial pressure decreased from, basal, 108 +/- 18 to 85 +/- 17, 76 +/- 16 and 69 +/- 18 mmHg (p < 0.0001), and central venous pressure decreased from, basal, 5.1 +/- 3 to 1.2 +/- 1, 0.4 +/- 1 and 0.8 +/- 2 mmHg (p < 0.001). Heart rate increased from, basal, 66 +/- 5 to 84 +/- 23, 111 +/- 29 and 114 +/- 13 b/min (p < 0.001). Following the administration of ANF the urine volume decreased from, basal, 261 +/- 145 to 195 +/- 72, 121 +/- 41 and 102 +/- 33 ml/h (p < 0.0041). The urinary sodium excretion rates decreased from, basal, 50 +/- 17 to 30 +/- 13, 22 +/- 10 and 19 +/- 10 mmol/h (p < 0.007), and the potassium excretion rates decreased from, basal, 43 +/- 19 to 39 +/- 16, 26 +/- 11 and 23 +/- 10 mmol/h (p < 0.0047). Endogenous clearance of creatinine (basal: 182 +/- 30 ml/min) did not change (260 +/- 84, 218 +/- 66 and 224 +/- 76 ml/h) following i.v. ANF. Inspite of this marked fluid retention, the hematocrit, expressed as relative change, was increased by 7.5%, 11.8% and 10.4%. In 2 calves we additionally measured an increased whole blood and plasma density possibly indicating increased liquid permeation to an extravascular compartment. Thus, in calves the hemodynamic effects of ANF are comparable to those seen in man. However, the failure of ANF to stimulate diuresis and natriuresis indicates a dissociation of hemodynamic and renal effects of ANF in calves.
在5头清醒的小牛（4月龄，体重94±14kg）中研究了静脉注射人心房利钠因子（α-hANF [99-126]）的作用，在实验前24小时以及实验3小时期间，维持恒定的液体（300ml/h）和钠摄入量。我们每隔1小时分别给予200微克、400微克和800微克的心房利钠因子。静脉注射后立即（+2分钟），肽的血清浓度从基础值13±3分别升至802±191（200微克）、1707±419（400微克）和3483±878pmol/l（800微克）（p<0.0001）。平均动脉压从基础值108±18分别降至85±17、76±16和69±18mmHg（p<0.0001），中心静脉压从基础值5.1±3分别降至1.2±1、0.4±1和0.8±2mmHg（p<0.001）。心率从基础值66±5分别升至84±23、111±29和114±13次/分钟（p<0.001）。给予心房利钠因子后，尿量从基础值261±145分别降至195±72、121±41和102±33ml/h（p<0.0041）。尿钠排泄率从基础值50±17分别降至30±13、22±10和19±10mmol/h（p<0.007），尿钾排泄率从基础值43±19分别降至39±16、26±11和23±10mmol/h（p<0.0047）。静脉注射心房利钠因子后，内源性肌酐清除率（基础值：182±30ml/min）未改变（260±84、218±66和224±76ml/h）。尽管有明显的液体潴留，但以相对变化表示的血细胞比容分别增加了7.5%、11.8%和10.4%。在2头小牛中，我们还测量到全血和血浆密度增加，这可能表明液体向血管外间隙的渗透增加。因此，在小牛中，心房利钠因子的血流动力学效应与人相似。然而，心房利钠因子未能刺激利尿和利钠表明小牛中心房利钠因子的血流动力学和肾脏效应存在分离。
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