从菰中分离到的一种假定新型杆状病毒的全基因组序列。

Complete genome sequence of a putative novel ilarvirus isolated from Eleocharis dulcis.

机构信息

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.

Biotechnology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China.

出版信息

Arch Virol. 2021 Dec;166(12):3477-3481. doi: 10.1007/s00705-021-05249-x. Epub 2021 Oct 4.

DOI:10.1007/s00705-021-05249-x

PMID:34608526

Abstract

The complete genomic sequence of a novel ilarvirus from Eleocharis dulcis, tentatively named "water chestnut virus A" (WCVA), was determined using next-generation sequencing (NGS) combined with reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The three genomic RNA components of WCVA were 3578 (RNA1), 2873 (RNA2), and 2073 (RNA3) nucleotides long, with four predicted open reading frames containing conserved domains and motifs typical of ilarviruses. Phylogenetic analysis of each predicted protein consistently placed WCVA in subgroup 4 of the genus Ilarvirus, together with prune dwarf virus, viola white distortion associated virus, Fragaria chiloensis latent virus, and potato yellowing virus. The genetic distances and lack of serological reaction to antisera against other ilarviruses suggest that WCVA is a novel member of the genus.

摘要

从菰中分离到一株新型杆状病毒，暂定名为“菱角病毒 A”（Water chestnut virus A，WCVA），通过下一代测序（next-generation sequencing，NGS）结合反转录聚合酶链反应（reverse transcription polymerase chain reaction，RT-PCR）和快速扩增 cDNA 末端（rapid amplification of cDNA ends，RACE）PCR，测定了该病毒的全基因组序列。WCVA 的三个基因组 RNA 成分分别为 3578（RNA1）、2873（RNA2）和 2073（RNA3）个核苷酸长，含有四个预测的开放阅读框，包含杆状病毒属典型的保守结构域和基序。每个预测蛋白的系统发育分析都将 WCVA 置于杆状病毒属第 4 亚组，与李痘病毒、堇菜花白色褶皱相关病毒、草莓潜隐病毒和马铃薯黄花病毒一起。遗传距离和对其他杆状病毒抗血清无血清学反应表明，WCVA 是该属的一个新成员。