Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - Campus Diadema. Rua Sao Nicolau, 210, 4º andar, 04023-900, Diadema, Brazil.
Curr Top Med Chem. 2018;18(4):300-311. doi: 10.2174/1568026618666180412154519.
The neglected tropical diseases (NTDs) are caused by several parasites, fungi, bacteria and viruses and affect more than one billion people in the world. The control and prevention against NTDs need implementation of alternative methods for testing new compounds against these diseases. For the implementation of alternative methods, it is necessary to apply the principles of replacement, reduction and refinement (the 3Rs) for the use of laboratory animals. Accordingly, the present review addressed a variety of alternative models to study the infections caused by protozoa and fungi. Overall, vertebrate and invertebrate models of fungal infection have been used to elucidate host-pathogen interactions. However, until now the insect model has not been used in protozoal studies as an alternative method, but there is interest in the scientific community to try new tools to screen alternative drugs to control and prevent protozoal infections.
被忽视的热带病（NTDs）由多种寄生虫、真菌、细菌和病毒引起，影响全球超过 10 亿人。控制和预防 NTDs 需要实施替代方法来测试针对这些疾病的新化合物。为了实施替代方法，有必要将实验室动物使用的替代、减少和优化（3Rs）原则应用于替代方法中。因此，本综述介绍了多种替代模型，用于研究原生动物和真菌引起的感染。总的来说，已经使用了脊椎动物和无脊椎动物的真菌感染模型来阐明宿主-病原体相互作用。然而，到目前为止，昆虫模型尚未被用于原生动物研究作为替代方法，但科学界有兴趣尝试新工具来筛选替代药物以控制和预防原生动物感染。
