MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge CB2 0QH, England.
Centre for Biological Sciences and the Institute for Life Sciences, University of Southampton, Southampton SO17 1BJ, England.
Acta Crystallogr D Struct Biol. 2018 Apr 1;74(Pt 4):256-263. doi: 10.1107/S2059798318005119. Epub 2018 Apr 6.
Coot is a graphics application that is used to build or manipulate macromolecular models; its particular forte is manipulation of the model at the residue level. The model-building tools of Coot have been combined and extended to assist or automate the building of N-linked glycans. The model is built by the addition of monosaccharides, placed by variation of internal coordinates. The subsequent model is refined by real-space refinement, which is stabilized with modified and additional restraints. It is hoped that these enhanced building tools will help to reduce building errors of N-linked glycans and improve our knowledge of the structures of glycoproteins.
Coot 是一个图形应用程序，用于构建或操作大分子模型；其特别擅长在残基水平上操作模型。Coot 的模型构建工具已被组合和扩展，以协助或自动化 N-连接聚糖的构建。模型通过添加单糖，通过内部坐标的变化来构建。随后的模型通过真实空间精修进行精修，并用修改和额外的约束进行稳定。希望这些增强的构建工具将有助于减少 N-连接聚糖的构建错误，并提高我们对糖蛋白结构的认识。
