Hackney Anthony C, Anderson Travis
Endocrine Section-Applied Physiology Laboratory, Department of Exercise & Sport Science. University of North Carolina, Chapel Hill, North Carolina, USA.
J Clin Mol Endocrinol. 2016;1(1). Epub 2016 Jan 8.
This study investigated the validity of salivary cortisol responses to reflect blood cortisol responses relative to the magnitude of change observed over time in the hormone. Male subjects (n=25) conducted four experimental sessions (ES) where blood (B) and saliva (S) were obtained before (PS) and after (PoS) a 30 min resting control, 40%, 60%, and 80% of maximal aerobic capacity (VO2max) exercise ES. B and S specimens were analyzed by standard biochemical procedures. Hormonal concentrations changes were assessed by using absolute delta (DA) values (PoS - PS) and percent change (PC) calculations ((PoS-PS)/PS × 100) for each B and S specimen. Subsequent DA and PC values were correlated (Pearson) for each B-S specimen pairing (n=100; n=25 × 4 ES). Results indicate the magnitude of change (PoS vs. PS) in S cortisol is more valid and strongly associated (p<0.001) with corresponding B changes (the "gold standard") when expressing the data as delta values using absolute hormonal concentrations as compared to percent change expression.
本研究调查了唾液皮质醇反应相对于该激素随时间观察到的变化幅度来反映血液皮质醇反应的有效性。男性受试者（n = 25）进行了四个实验环节（ES），在30分钟静息对照、最大有氧能力（VO2max）的40%、60%和80%的运动实验环节前后分别采集血液（B）和唾液（S）样本。通过标准生化程序分析B和S样本。使用每个B和S样本的绝对差值（DA）值（实验后 - 实验前）和百分比变化（PC）计算（（实验后 - 实验前）/实验前×100）来评估激素浓度变化。对每个B - S样本配对（n = 100；n = 25×4个实验环节）的后续DA和PC值进行相关性分析（Pearson）。结果表明，当使用绝对激素浓度以差值表示数据时，与百分比变化表示相比，S皮质醇的变化幅度（实验后与实验前相比）更有效，并且与相应的B变化（“金标准”）密切相关（p<0.001）。
