Department of Environmental Engineering, National Ilan University, Yilan 260, Taiwan.
Department of Environmental Engineering, National Ilan University, Yilan 260, Taiwan.
Bioresour Technol. 2018 Aug;261:44-51. doi: 10.1016/j.biortech.2018.04.010. Epub 2018 Apr 5.
This study aimed to establish a mathematical modeling to evaluate the inhibitory effect of phenolic derivatives on acetone-butanol-ethanol (ABE) fermentation by Clostridium saccharoperbutylacetonicum N1-4. Vanillin, 4-hydroxybenzoic acid, and syringaldehyde were selected to represent guaiacyl, hydroxyphenyl, and syringyl phenols, respectively, to be examined in a series of fed-batch experiments. Results show the presence of phenolic derivatives blocked the pathway of the assimilation of organic acids and reduced cell growth and glucose utilization. The inhibition model projected that the levels of 0.13, 0.14, and 0.04 g L for vanillin, 4-hydroxybenzoic acid, and syringaldehyde, respectively, resulted in 25% inhibition of butanol production, whereas 100% inhibition was predicted at the levels of 4.94, 4.37, and 4.20 g L for vanillin, 4-hydroxybenzoic acid, and syringaldehyde, respectively. Syringaldehyde was more toxic than the other two compounds. The established model described that the phenolic compounds derived from different phenyl propane monomers of lignin severely obstructed biobutanol production.
本研究旨在建立数学模型来评估酚类衍生物对丙酮丁醇乙醇（ABE）发酵的抑制作用，使用丙酮丁醇梭菌（Clostridium saccharoperbutylacetonicum N1-4）作为模型菌。选取香草醛、对羟基苯甲酸和丁香醛分别代表愈创木基、羟基苯基和丁香基酚，在一系列分批补料实验中进行考察。结果表明，酚类衍生物的存在阻断了有机酸的同化途径，从而降低了细胞生长和葡萄糖的利用。抑制模型预测，香草醛、对羟基苯甲酸和丁香醛的浓度分别为 0.13、0.14 和 0.04 g/L 时，丁醇产量的抑制率为 25%，而当香草醛、对羟基苯甲酸和丁香醛的浓度分别达到 4.94、4.37 和 4.20 g/L 时，丁醇产量的抑制率达到 100%。丁香醛比其他两种化合物的毒性更大。所建立的模型表明，木质素中不同苯基丙烷单体衍生的酚类化合物严重阻碍了生物丁醇的生产。
