Qiao Jing, Li Haili, Tang Yun, Song Wenjing, Rong Bei, Yang Songlin, Wu Yuan, Yan Xiaoming
Department of Ophthalmology, Peking University First Hospital, Beijing, 100034, China.
BMC Ophthalmol. 2018 Apr 13;18(1):94. doi: 10.1186/s12886-018-0760-z.
To investigate use of collagenase type II for generating a rabbit model of corneal ectasia.
Ten New Zealand white rabbits were used with right eyes treated as the experimental group and left eyes treated as the control group. After epithelial debridement, a collagenase type II solution (200 μL of 5 mg/mL) was applied in the experimental group at room temperature (24 °C) for 30 min, and a 200 μL solution without collagenase was applied in the control group. Slit-lamp microscopy, the mean keratometry (Km), and central cornea thickness (CCT) were examined before and after the procedure. Corneas were obtained on day 14 for biomechanical evaluation.
No obvious inflammatory reaction was observed in all eyes after the procedure. A statistically significant increase in Km (1.54 ± 1.29D vs - 0.82 ± 0.44D at day7 and 0.89 ± 0.89D vs - 2.11 ± 1.02D at day14) and a statistically significant decrease in CCT (- 23.10 ± 12.17 μm vs 6.20 ± 16.51 μm at day7 and - 16.10 ± 10.46 μm vs 11.60 ± 0.88 μm at day14) were observed in the experimental group compared with the control group. The mean stresses and elastic modulus at 5%, 10%, 15%, and 20% deformities in the experimental group decreased and the differences in elastic modulus between the two groups were statistically significant at 10% and 15% deformities.
Collagenase type II treatment results in mimic KC with increased corneal keratometry and corneal thinning and a lower elastic modulus. An animal model for corneal ectasia can be generated by treatment with collagenase type II.
研究使用II型胶原酶建立兔角膜扩张模型。
选用10只新西兰白兔，右眼作为实验组，左眼作为对照组。在角膜上皮清创后，实验组在室温（24℃）下应用II型胶原酶溶液（200μL，5mg/mL）30分钟，对照组应用200μL不含胶原酶的溶液。在操作前后进行裂隙灯显微镜检查、平均角膜曲率（Km）和中央角膜厚度（CCT）测量。在第14天获取角膜进行生物力学评估。
术后所有眼均未观察到明显炎症反应。与对照组相比，实验组在第7天Km有统计学意义的增加（1.54±1.29D vs -0.82±0.44D），在第14天Km也有统计学意义的增加（0.89±0.89D vs -2.11±1.02D）；在第7天CCT有统计学意义的降低（-23.10±12.17μm vs 6.20±16.51μm），在第14天CCT也有统计学意义的降低（-16.10±10.46μm vs 11.60±0.88μm）。实验组在5%、10%、15%和20%变形时的平均应力和弹性模量降低，两组在10%和15%变形时弹性模量的差异有统计学意义。
II型胶原酶治疗可导致类似圆锥角膜的表现，角膜曲率增加、角膜变薄且弹性模量降低。通过II型胶原酶治疗可建立角膜扩张动物模型。
