Shanghai Key Laboratory of Pancreatic Diseases & Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 200080 Shanghai, PR China.
Department of Pathology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 200080 Shanghai, PR China.
Pancreatology. 2018 Jun;18(4):438-445. doi: 10.1016/j.pan.2018.03.005. Epub 2018 Apr 6.
An increasing number of reports have demonstrated that there is an association between the presence of pathogenic microorganisms and pancreatic cancer. However, the role of the duodenal microbiota in pancreatic carcinogenesis remains unknown. In this study, duodenal mucosal microbiota was analyzed in 14 patients with pancreatic head cancer and 14 healthy controls using 16S rRNA gene pyrosequencing methods. Plasma endotoxin activity and the concentrations of the proinflammatory cytokine IL-6 and C-reactive protein (CRP) were measured in blood samples. The urea breath test was used to detect Helicobacter pylori infections. Endoscopic duodenal mucosal biopsies were evaluated by histological examinations. Statistical comparisons of inflammatory factors revealed significantly higher levels of CRP and IL-6 in the pancreatic cancer group as compared to healthy controls. Patients with pancreatic cancer also had a higher incidence of H. pylori infections and showed mucosal changes, including villous abnormalities and diffuse inflammatory cell infiltration in the lamina propria. The sequences analysis showed that based on linear discriminant analysis effect size (LEfSe) analysis at the genus level, Acinetobacter, Aquabacterium, Oceanobacillus, Rahnella, Massilia, Delftia, Deinococcus, and Sphingobium were more abundant in the duodenal mucosa of pancreatic cancer patients, whereas the duodenal microbiotas of healthy controls were enriched with Porphyromonas, Paenibacillus, Enhydrobacter, Escherichia, Shigella, and Pseudomonas. These results reveal a picture of duodenal microbiota in pancreatic head cancer patients that could be useful in future trials investigating the role of gut microbiota in pancreatic cancer.
越来越多的报告表明，致病性微生物的存在与胰腺癌之间存在关联。然而，十二指肠微生物群在胰腺癌发生中的作用尚不清楚。在这项研究中，我们使用 16S rRNA 基因焦磷酸测序方法分析了 14 例胰头癌患者和 14 例健康对照者的十二指肠黏膜微生物群。在血液样本中测量血浆内毒素活性和促炎细胞因子 IL-6 和 C 反应蛋白 (CRP) 的浓度。使用尿素呼气试验检测幽门螺杆菌感染。通过组织学检查评估内镜十二指肠黏膜活检。对炎症因子进行统计学比较显示，与健康对照组相比，胰腺癌组的 CRP 和 IL-6 水平显著更高。胰腺癌患者还具有更高的幽门螺杆菌感染发生率，并表现出黏膜变化，包括绒毛异常和固有层弥漫性炎症细胞浸润。序列分析显示，基于线性判别分析效应大小 (LEfSe) 分析在属水平上，不动杆菌属、Aquabacterium 属、海洋杆菌属、Rahnella 属、马塞利亚菌属、Delftia 属、Deinococcus 属和鞘氨醇单胞菌属在胰腺癌患者的十二指肠黏膜中更为丰富，而健康对照组的十二指肠微生物群则富含卟啉单胞菌属、类芽孢杆菌属、 Enhydrobacter 属、大肠杆菌属、志贺菌属和假单胞菌属。这些结果揭示了胰头癌患者十二指肠微生物群的情况，这可能有助于未来研究肠道微生物群在胰腺癌中的作用。
