Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany.
Microbial Interactions and Processes (MINP) Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Gut. 2018 Feb;67(2):216-225. doi: 10.1136/gutjnl-2016-312904. Epub 2016 Dec 5.
Patients infected with develop chronic gastritis with a subgroup progressing to further complications. The role of microbiota from the oral cavity swallowed with saliva and either transiting the stomach or persisting in the gastric mucosa is uncertain. It is also not known whether the bacterial community differs in luminal and mucosal niches. A key question is whether influences the bacterial communities of gastroduodenal niches.
Saliva, gastric and duodenal aspirates as well as gastric and duodenal biopsies were collected during oesophagogastroduodenoscopy from 24 patients (m:9, f:15, mean age 52.2±SD 14.5 years). RNA was extracted and the V1-V2 region of the retrotranscribed bacterial 16S rRNA amplified and sequenced on the Illumina MiSeq platform.
Overall, 687 bacterial phylotypes that belonged to 95 genera and 11 phyla were observed. Each individual comprised a unique microbiota composition that was consistent across the different niches. However, the stomach fluid enriched for specific microbiota components. spp were shown to dominate the mucosa-associated community in the stomach, and to significantly influence duodenal and oral communities.
The detailed analysis of the active global bacterial communities from the five distinct sites of the upper GI tract allowed for the first time the differentiation between host effects and the influence of sampling region on the bacterial community. The influence of spp on the global community structures is striking.
感染 的患者会发生慢性胃炎，其中一部分患者会进一步发展为其他并发症。由口腔菌群组成的物质会随着唾液进入人体，这些物质可能会穿过胃部或者停留在胃黏膜中，其具体作用尚不清楚。此外，人们也不清楚这些菌群在胃腔和黏膜腔隙中的分布是否存在差异。其中一个关键问题是 是否会影响胃十二指肠腔隙中的细菌群落。
研究人员通过胃镜从 24 名患者（男 9 名，女 15 名，平均年龄 52.2±14.5 岁）的口腔、胃和十二指肠抽吸物以及胃和十二指肠活检组织中采集了唾液、胃液和十二指肠液样本，并提取了 RNA，然后使用 Illumina MiSeq 平台对逆转录细菌 16S rRNA 的 V1-V2 区进行扩增和测序。
总的来说，观察到了 687 个细菌种属，它们隶属于 95 个属和 11 个门。每个个体都具有独特的微生物群落组成，并且这种组成在不同的腔隙中是一致的。然而，胃液中富含特定的微生物群落成分。 spp 在胃黏膜相关群落中占据主导地位，并且显著影响十二指肠和口腔群落。
对五个不同上消化道部位的活跃全细菌群落进行详细分析，首次区分了宿主效应和采样部位对细菌群落的影响。 spp 对全球群落结构的影响是显著的。
