Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita city, Osaka, Japan.
Division of Health Sciences, Department of Molecular Pathology, Graduate School of Medicine, Osaka University, Suita City, Osaka, Japan.
Mol Cancer Ther. 2018 Jul;17(7):1613-1622. doi: 10.1158/1535-7163.MCT-17-0788. Epub 2018 Apr 13.
Minimally invasive treatment is getting more and more important in an aging society. The purpose of this study was to explore the possibility of ICG loaded on super carbonate apatite (sCA) nanoparticles as a novel photodynamic therapy (PDT) against cancers. Using colon cancer cells, ICG uptake and anti-tumor effects were examined between the treatments of ICG and sCA-ICG. Reactive oxygen species (ROS) production and temperature rise were also evaluated to explore the underlying mechanism. Atomic force microscopy revealed that the size of sCA-ICG ranged from 10 to 20 nm. In aqueous solution with 0.5% albumin, the temperature increase after laser irradiation was 27.1°C and 23.1°C in sCA-ICG and ICG, respectively (control DW: 5.7°C). A significant increase in ROS generation was noted in cell cultures treated with sCA-ICG plus irradiation compared with those treated with ICG plus irradiation ( < 0.01). Uptake of ICG in the tumor cells significantly increased in sCA-ICG compared with ICG and The fluorescence signals of ICG in the tumor, liver, and kidney faded away in both treatments by 24 hours. Finally, the HT29 tumors treated with sCA-ICG followed by irradiation exhibited drastic tumor growth retardation ( < 0.01), whereas irradiation of tumors after injection of ICG did not inhibit tumor growth. This study shows that sCA is a useful vehicle for ICG-based PDT. Quick withdrawal of ICG from normal organs is unique to sCA-ICG and contrasts with the other nanoparticles remaining in normal organs for a long time. .
在老龄化社会中，微创治疗变得越来越重要。本研究旨在探索将 ICG 负载在超碳酸磷灰石（sCA）纳米粒子上作为一种新型光动力疗法（PDT）治疗癌症的可能性。使用结肠癌细胞，研究了 ICG 和 sCA-ICG 处理之间的 ICG 摄取和抗肿瘤作用。还评估了活性氧（ROS）的产生和温升，以探讨其潜在机制。原子力显微镜显示 sCA-ICG 的尺寸范围为 10 至 20nm。在含有 0.5%白蛋白的水溶液中，激光照射后 sCA-ICG 和 ICG 的升温分别为 27.1°C 和 23.1°C（对照 DW：5.7°C）。与单独用 ICG 处理加照射的细胞相比，用 sCA-ICG 处理加照射的细胞中 ROS 生成显著增加（<0.01）。与 ICG 相比，sCA-ICG 中肿瘤细胞对 ICG 的摄取明显增加。在两种处理中，肿瘤、肝和肾中的 ICG 荧光信号在 24 小时内消失。最后，用 sCA-ICG 处理后再照射的 HT29 肿瘤表现出明显的肿瘤生长抑制（<0.01），而注射 ICG 后照射肿瘤则不能抑制肿瘤生长。本研究表明 sCA 是 ICG 基 PDT 的一种有用载体。sCA-ICG 能迅速从正常器官中排出 ICG，这与其他纳米粒子在正常器官中长时间滞留形成对比。
