Chemical Engineering Department, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran.
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
J Drug Target. 2024 Aug;32(7):820-837. doi: 10.1080/1061186X.2024.2358511. Epub 2024 Jun 19.
Indocyanine Green (ICG) as an agent for photodynamic therapy (PDT) of melanoma cancer has low quantum yield, short circulation half-life, poor photo-stability, and tendency to aggregation.
N-doped carbon quantum dot (CQD) nanoparticle was applied to encapsulate ICG and overcome ICG obstacle in PDT with simultaneous cell imaging property.
CQD was prepared using hydrothermal method. Cell culture study and assessments on C57BL/6 mice containing melanoma cancer cells was performed.
Results showed that CQD size slightly enhanced from 24.55 nm to 42.67 nm after ICG loading. Detection of reactive oxygen species (ROS) demonstrated that CQD improved ICG photo-stability and ROS generation capacity upon laser irradiation. Cell culture study illustrated that ICG@CQD could decrease survival rate of melanoma cancer cells of B16F10 cell line from 48% for pure ICG to 28% for ICG@CQD. Confocal microscopy images approved more cellular uptake and more qualified cell imaging ability of ICG@CQD. assessments displayed obvious inhibitory effect of tumor growth for ICG@CQD in comparison to free ICG on the C57BL/6 mice. fluorescence images confirmed that ICG@CQD accumulates remarkably more than free ICG in tumor region. Finally, ICG@CQD was proposed as an innovative nanocarrier for PDT and diagnosis.
吲哚菁绿（ICG）作为光动力疗法（PDT）治疗黑色素瘤的试剂，量子产率低、循环半衰期短、光稳定性差，容易聚集。
氮掺杂碳量子点（CQD）纳米颗粒被应用于包裹 ICG，以克服 ICG 在 PDT 中存在的障碍，同时具有细胞成像特性。
采用水热法制备 CQD。在 C57BL/6 小鼠中进行细胞培养研究和含有黑色素瘤癌细胞的评估。
结果表明，ICG 负载后 CQD 的尺寸从 24.55nm 略微增加到 42.67nm。活性氧（ROS）的检测表明，CQD 提高了 ICG 的光稳定性和激光照射下 ROS 的产生能力。细胞培养研究表明，ICG@CQD 可以将 B16F10 细胞系黑色素瘤癌细胞的存活率从纯 ICG 的 48%降低到 ICG@CQD 的 28%。共聚焦显微镜图像证实了 ICG@CQD 具有更多的细胞摄取和更高质量的细胞成像能力。在 C57BL/6 小鼠中的评估显示，与游离 ICG 相比，ICG@CQD 对肿瘤生长具有明显的抑制作用。荧光图像证实，ICG@CQD 在肿瘤区域的积累明显多于游离 ICG。最后，ICG@CQD 被提议作为一种用于 PDT 和诊断的创新纳米载体。
