State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xian Nong Tan Street, Beijing, 100050, China.
Department of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Beilishi Rd, Beijing, 100037, China.
Chem Asian J. 2018 Jun 4;13(11):1432-1437. doi: 10.1002/asia.201800530. Epub 2018 May 17.
Energy transfer between fluorescent dyes and quenchers is widely used in the design of light-up probes. Although dual quenchers are more effective in offering lower background signals and higher turn-on ratios than one quencher, such probes are less explored in practice as they require both quenchers to be within the proximity of the fluorescent core. In this contribution, we utilized intramolecular motion and photoinduced electron transfer (PET) as quenching mechanisms to build super-quenched light-up probes based on fluorogens with aggregation-induced emission. The optimized light-up probe possesses negligible background and is able to detect not only free formaldehyde (FA) but also polymeric FA, with an unprecedented turn-on ratio of >4900. We envision that this novel dual quenching strategy will help to develop various light-up probes for analyte sensing.
能量转移在荧光染料和猝灭剂之间的广泛应用于设计点亮探针。虽然双猝灭剂比一个猝灭剂更有效地提供更低的背景信号和更高的开启比，但由于它们需要两个猝灭剂都在荧光核心的附近，因此在实践中这种探针的应用较少。在本贡献中，我们利用分子内运动和光诱导电子转移 (PET) 作为猝灭机制，基于具有聚集诱导发射的荧光团构建了超猝灭点亮探针。优化后的点亮探针具有可以忽略不计的背景，不仅能够检测游离甲醛 (FA)，还能够检测聚合态的 FA，开启比超过 4900。我们设想，这种新的双猝灭策略将有助于开发用于分析物检测的各种点亮探针。
