Department of Neurology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China (mainland).
Med Sci Monit. 2018 Apr 14;24:2235-2243. doi: 10.12659/msm.906533.
BACKGROUND Ischemic stroke, featuring high incidence, morbidity, and mortality, is one of the three major diseases troubling human beings. The purpose of the study was to examine the impact of early high-protein diet on neurofunctional recovery in rats with ischemic stroke as well as their cerebral infarct areas and molecular expressions of oxidative stress. MATERIAL AND METHODS The middle cerebral artery occlusion model (MCAO) was established, and 48 adult, male Sprague Dawley (SD) rats of clean grade aged seven to eight months (250-280 g body weight) were randomized into four groups: the MCAO group with high-protein diet (MH), the MCAO group with standard-protein diet (MS), the sham group with high-protein diet (SH), and the sham group with standard-protein diet (SS). High-protein diet intervention started on the first day of the surgery, and the rats' body weights and their neurological deficit scores were measured on each postoperative day while the scores of motors coordination and balance ability were recorded every other day. In addition, their cerebral infant areas and the molecular expressions of oxidative stress injuries were detected as well. RESULTS Compared to the MS group, the rats in the MH group gained faster weight growth (p<0.05), presented significantly lower neurological impairment scores (p<0.05), remarkably improved motor coordination and balance ability (p<0.05) as well as showed smaller cerebral infarct areas (p<0.05), increased expression of SOD (superoxide dismutase), and reduced expressions of MDA (malondialdehyde) and iNOS (inducible nitric oxide synthase). However, there was no significant difference between the SS group and the SH group (p>0.05). CONCLUSIONS Early high-protein diet facilitates the recovery of body weights and neurological functions as well the reduction of the cerebral infarct areas of rats, thus alleviating ischemic stroke-caused oxidative stress injuries.
缺血性脑卒中具有发病率、致残率和死亡率高的特点，是困扰人类的三大疾病之一。本研究旨在探讨早期高蛋白饮食对缺血性脑卒中大鼠神经功能恢复及其脑梗死面积和氧化应激分子表达的影响。
建立大脑中动脉闭塞模型（MCAO），将 48 只清洁级雄性成年 Sprague Dawley（SD）大鼠随机分为 4 组：MCAO 高蛋白饮食组（MH）、MCAO 标准蛋白饮食组（MS）、假手术高蛋白饮食组（SH）和假手术标准蛋白饮食组（SS）。高蛋白饮食干预从手术第一天开始，术后每天测量大鼠体重和神经功能缺损评分，每隔一天记录运动协调和平衡能力评分。此外，还检测了脑梗死面积和氧化应激损伤的分子表达。
与 MS 组相比，MH 组大鼠体重增长更快（p<0.05），神经功能缺损评分明显降低（p<0.05），运动协调和平衡能力显著改善（p<0.05），脑梗死面积较小（p<0.05），SOD（超氧化物歧化酶）表达增加，MDA（丙二醛）和 iNOS（诱导型一氧化氮合酶）表达减少。然而，SS 组和 SH 组之间没有显著差异（p>0.05）。
早期高蛋白饮食促进了大鼠体重和神经功能的恢复，减少了脑梗死面积，从而减轻了缺血性脑卒中引起的氧化应激损伤。
