Tannahill Victoria J, Cogan Tristan, Allen Kate, Acutt Elizabeth, Busschers Evita
School of Veterinary Sciences, University of Bristol, Bristol, United Kingdom.
Vet Surg. 2018 May;47(4):572-577. doi: 10.1111/vsu.12793. Epub 2018 Apr 14.
To determine the efficacy and dermal tolerance of a novel alcohol-based skin antiseptic (ABSA) in horses.
Experimental study.
Systemically healthy horses (n = 25) with no history or clinical signs of skin disease.
Four clipped sites on the abdomen were randomly assigned to a skin preparation protocol: saline (negative control; NC), chlorhexidine gluconate followed by isopropyl alcohol (positive control; PC), saline followed by the ABSA (ABSA A), or a commercially available horse shampoo followed by the ABSA (ABSA B). Microbiological swabs were obtained from each site and cultured on MacConkey and mannitol salt agar plates. Colony-forming units were counted 18-24 hours later. All sites were scored for signs of skin reaction before, immediately after, 1 hour after, and 24 hours after skin preparation.
The PC, ABSA A, and ABSA B methods reduced skin microbial burden compared with the NC method (P < .001), but no difference was detected between antiseptic products. Preparation time did not differ between ABSA A and ABSA B methods (P = 0.108); both were faster than the PC method (P < 0.001 for both). Skin reactions were most abundant 24 hours after skin preparation (30.5%), but there was no significant association with antiseptic used, and no horses required veterinary treatment.
The ABSA preparations tested in this study were as effective and well tolerated as a chlorhexidine gluconate-based method, but required less time in healthy horses.
The ABSA tested here provides an efficacious, fast-acting, and well-tolerated alternative to achieve skin antisepsis in healthy horses. These results justify further investigation in clinical cases.
确定一种新型酒精基皮肤消毒剂（ABSA）对马匹的疗效和皮肤耐受性。
实验研究。
无全身性疾病且无皮肤病病史或临床症状的健康马匹（n = 25）。
腹部的四个剪毛部位随机分配接受一种皮肤准备方案：生理盐水（阴性对照；NC）、葡萄糖酸洗必泰后接异丙醇（阳性对照；PC）、生理盐水后接ABSA（ABSA A），或市售马用洗发水后接ABSA（ABSA B）。从每个部位获取微生物拭子，并在麦康凯和甘露醇盐琼脂平板上培养。18 - 24小时后计数菌落形成单位。在皮肤准备前、后立即、1小时后和24小时后对所有部位的皮肤反应迹象进行评分。
与NC方法相比，PC、ABSA A和ABSA B方法降低了皮肤微生物负荷（P < 0.001），但在消毒剂产品之间未检测到差异。ABSA A和ABSA B方法的准备时间无差异（P = 0.108）；两者均比PC方法快（两者P < 0.001）。皮肤反应在皮肤准备后24小时最为常见（30.5%），但与所用消毒剂无显著关联，且没有马匹需要兽医治疗。
本研究中测试的ABSA制剂与基于葡萄糖酸洗必泰的方法一样有效且耐受性良好，但在健康马匹中所需时间更少。
此处测试的ABSA为健康马匹实现皮肤消毒提供了一种有效、快速起效且耐受性良好的替代方法。这些结果证明在临床病例中进一步研究是合理的。
