Zimecki M, Kapp J A
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Arch Immunol Ther Exp (Warsz). 1987;35(3):361-70.
GAT-specific helper T cell lines were shown to bind GAT, coupled to sepharose or sheep erythrocytes. The binding of T cell lines to GAT was specific, since forming of rosettes with GAT-SRBC was inhibited by pretreatment of T cells with free GAT or anti-idiotypic antibodies. Surface iodination of cells and purification of cell lysates on GAT-sepharose, revealed the presence of a 66 KD molecule and a smaller, 33 KD fragment. The proteins could be eluted specifically from GAT-sepharose with a free GAT, or nonspecifically with SDS. The 66 KD molecule could not be reduced by 5% 2-mercaptoethanol. In addition, it was found that the GAT-binding molecule did not bind to the antibodies against the heterodimer (T-cell receptor). The results suggest that the antigen receptor for GAT on helper T cells and inducer suppressor T cells may be related. The GAT-binding molecule may be loosely associated with the heterodimer.
已证明，GAT特异性辅助性T细胞系可与偶联到琼脂糖珠或绵羊红细胞上的GAT结合。T细胞系与GAT的结合具有特异性，因为用游离GAT或抗独特型抗体预处理T细胞可抑制GAT-绵羊红细胞玫瑰花结的形成。对细胞进行表面碘化，并在GAT-琼脂糖珠上纯化细胞裂解物，结果显示存在一个66千道尔顿的分子和一个较小的33千道尔顿的片段。这些蛋白质可以用游离GAT从GAT-琼脂糖珠上特异性洗脱，或者用SDS非特异性洗脱。66千道尔顿的分子不能被5%的2-巯基乙醇还原。此外，还发现GAT结合分子不与针对异二聚体（T细胞受体）的抗体结合。结果表明，辅助性T细胞和诱导性抑制性T细胞上的GAT抗原受体可能相关。GAT结合分子可能与异二聚体松散结合。
