FDA, Gulf Coast Seafood Laboratory, Dauphin Island, AL 36528, USA; MIN Faculty, Chemistry Department, Institute for Biochemistry and Molecular Biology, University of Hamburg, Germany.
FDA, OLSS, Silver Spring, MD 20993, USA.
Food Chem. 2018 Aug 30;258:129-136. doi: 10.1016/j.foodchem.2018.03.054. Epub 2018 Mar 14.
This study investigates the enrichment of aptamers targeting the norovirus protruding domain in the presence of foods often associated with norovirus outbreaks. The goal is to explore if and how the presence of food alters in vitro selection of aptamers and target binding of the enriched oligonucleotides. Our study demonstrates that the introduction of food to SELEX (systematic evolution of ligands by exponential enrichment) is either detrimental to enrichment of oligonucleotides with target-specific binding, or facilitates enrichment of non-target-specific oligonucleotides. Moreover, a relationship between target binding of enriched oligonucleotides in presence of food and their selection condition was not observed. Our findings also suggest that a pathogen specific aptamer with application in food does not need to be selected in presence of the particular food, but may require properties beyond high affinity and selectivity to be applied for pathogen extraction and detection in undiluted food matrices.
本研究调查了在常与诺如病毒爆发相关的食物存在的情况下，针对诺如病毒突出结构域的适体的富集情况。目的是探索食物的存在是否以及如何改变体外适体选择和富集的寡核苷酸的靶结合。我们的研究表明，将食物引入 SELEX（配体指数富集的系统进化）中，要么不利于具有靶特异性结合的寡核苷酸的富集，要么有利于非靶特异性寡核苷酸的富集。此外，在存在食物的情况下，富集的寡核苷酸的靶结合与它们的选择条件之间没有观察到关系。我们的研究结果还表明，应用于食品的病原体特异性适体不一定需要在特定的食物存在下进行选择，但可能需要除高亲和力和选择性之外的特性，才能应用于未稀释的食物基质中的病原体提取和检测。
