Wang Jin
Research and Development Center for Taste and Odor Sensing, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, Japan.
Anal Biochem. 2018 Jun 1;550:49-53. doi: 10.1016/j.ab.2018.04.011. Epub 2018 Apr 12.
A near infrared (NIR) optical biosensor based on peptide functionalized single-walled carbon nanotubes (SWCNTs) hybrids for 2,4,6-trinitrotoluene (TNT) explosive detection was developed. The TNT binding peptide was directly anchored on the sidewall of the SWCNTs using the π-π interaction between the aromatic amino acids and SWCNTs, forming the peptide-SWCNTs hybrids for near infrared absorption spectra measurement. The evidence of the morphology of peptide-SWCNTs hybrids was obtained using atomic force microscopy (AFM). The results demonstrated that peptide-SWCNTs hybrids based NIR optical biosensor exhibited sensitive and highly selective for TNT explosive determination, addressing a promising optical biosensor for security application.
开发了一种基于肽功能化单壁碳纳米管（SWCNTs）杂化物的近红外（NIR）光学生物传感器，用于2,4,6-三硝基甲苯（TNT）爆炸物检测。利用芳香族氨基酸与SWCNTs之间的π-π相互作用，将TNT结合肽直接锚定在SWCNTs的侧壁上，形成用于近红外吸收光谱测量的肽-SWCNTs杂化物。使用原子力显微镜（AFM）获得了肽-SWCNTs杂化物形态的证据。结果表明，基于肽-SWCNTs杂化物的近红外光学生物传感器对TNT爆炸物测定具有灵敏性和高选择性，是一种有前景的用于安全应用的光学生物传感器。
