Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, PR China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
Bioresour Technol. 2018 Jul;260:427-431. doi: 10.1016/j.biortech.2018.03.109. Epub 2018 Mar 30.
The constitutively expression system for P. putida nitrilase was firstly constructed to improve the nicotinic acid production and reduce the production costs. High density culture strategy was employed to enhance the biomass and nitrilase production of recombinant strain. The total nitrilase activity reached up to 654 U·mL without the induction. 541 g·L nicotinic acid was accumulated via fed batch mode of substrate feeding through 290 min of conversion.
首先构建了组成型表达系统以提高产烟酸假单胞菌腈水解酶的产量并降低生产成本。采用高密度培养策略来提高重组菌的生物量和腈水解酶的产量。在未诱导的情况下，总腈水解酶活性达到了 654 U·mL。通过底物补料的方式进行分批补料转化 290 min，积累了 541 g·L 的烟酸。
