利用高诱导的Globorhodococcus NHB-2 腈酶介导的生物过程从 3-氰基吡啶合成烟酸。
An improved nitrilase-mediated bioprocess for synthesis of nicotinic acid from 3-cyanopyridine with hyperinduced Nocardia globerula NHB-2.
机构信息
Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171005, India.
出版信息
J Ind Microbiol Biotechnol. 2011 Sep;38(9):1235-43. doi: 10.1007/s10295-010-0902-7. Epub 2010 Nov 21.
Nitrilase of Nocardia globerula NHB-2 was induced by short-chain aliphatic nitriles (valeronitrile > isobutyronitrile > butyronitrile > propionitrile) and exhibited activity towards aromatic nitriles (benzonitrile > 3-cyanopyridine > 4-cyanopyridine > m-tolunitrile > p-tolunitrile). Hyperinduction of nitrilase (6.67 U mg (DCW) (-1), 18.7 U mL(-1)) was achieved in short incubation time (30 h, 30°C) through multiple feeding of isobutyronitrile in the growth medium. The nitrilase of this organism exhibits both substrate and product inhibition effects. In a fed batch reaction at 1 L scale using hyperinduced resting cells corresponding to 10 U mL(-1) nitrilase activity (1.5 mg(DCW) mL(-1)), a total of 123.11 g nicotinic acid was produced at a rate of 24 g h(-1) g (DCW) (-1).
球孢诺卡氏菌 NHB-2 的腈酶可被短链脂肪族腈（戊腈＞异丁腈＞丁腈＞丙腈）诱导，且对芳香族腈（苯甲腈＞3-氰基吡啶＞4-氰基吡啶＞间甲苯腈＞对甲苯腈）具有活性。在生长培养基中多次补加异丁腈，可在短时间（30 h，30°C）内实现腈酶的超诱导（6.67 U mg（DCW）（-1），18.7 U mL（-1））。该酶表现出底物和产物抑制效应。在 1 L 规模的流加分批反应中，使用对应 10 U mL（-1）腈酶活性（1.5 mg（DCW）mL（-1））的超诱导静止细胞，以 24 g h（-1）g（DCW）（-1）的速率生产了 123.11 g 烟酸。
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